纳米金修饰玻碳电极测定邻苯二酚
Electrochemical Determination of Catechol with Nano-gold Modified Glassy Carbon Electrode
摘 要
采用恒电位沉积方法将HAuCl4直接还原成纳米金并修饰于玻碳电极表面,制备了对邻苯二酚具有电催化氧化作用的纳米金修饰电极.邻苯二酚在该修饰电极上发生一可逆的氧化还原反应.在磷酸盐缓冲溶液(pH 7.5)中,当邻苯二酚的浓度为3.0×10-3mol·L-1时,与裸玻碳电极相比,其Epa负位移了170 mV,Epc正位移了50 mV,ΔE下降为60 mV,且峰电流显著增大,氧化峰电流与邻苯二酚浓度在5.0×10-6~4.2×10-3mol·L-1范围内呈线性关系,相关系数为0.997 6,检出限(3σ)为5.0×10-7mol·L-1.在浓度为5.0×10-4mol·L-1测得RSD(n=10)为2.9%,回收率在98.0%~101.0%之间.
邻苯二酚
电催化氧化
Nano-gold modified glassy carbon electrode
Catechal
Electrocatalytic oxidation
Abstract
A nanogold modified electrode behaving with electrocatalytic oxidation toward catechol was prepared by constant potential deposition of nanogold which was obtained by direct reduction of HAuCl4 onto the glassy carbon electrode.The electrochemical behavior of the modified electrode was studied by using a tri-electrode system with the modified electrode (or the bare glassy carbon electrode) as working electrode,SCE as reference electrode and Pt-wire as counter electrode.It was found that in a phosphate buffer solution of pH 7.5,a well-shaped redox peak of catechol (3.0×10-3mol·L-1 solution) was observed at the nanogold modified electrode,with Epa=140 mV,Epc=80 mV,Ipa/Ipc≈1.As compared with the bare glassy carbon electrode,a negative shift of Epa of 170 mV,a positive shift of Epc of 50 mV,a decrease of ΔE of 60 mV and a remarkable increase of peak current were obtained,denoting that the nano-gold modified electrode showed a distinct catalytic oxidation toward catechal,which was easier to be involved in the reversible redox reaction.Linear relationship was kept between Ipa and concentration of catechal in the range of 5.0×10-6-4.2×10-3mol·L-1,with a correlation coefficient of 0.997 6.Detection limit (3σ) of this method was found to be 5.0×10-7mol·L-1.RSD′s (n=10) tested at 5.0×10-4mol·L-1 was 2.9%.The recoveries tested gave values in the range of 98.0%-101.0%.
中图分类号 O657.31
所属栏目 工作简报
基金项目 国家自然科学基金(29705001),教育部优秀青年教师资助计划及重庆市自然科学基金(20027477)资助
收稿日期 2006/1/2
修改稿日期
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备注张英(1980-),女,四川人,硕士研究生,主要从事电化学及生物传感器的研究.
引用该论文: ZHANG Ying,YUAN Ruo,CHAN Ya-qin,FU Ying-zi,ZHUO Ying,LI Xue-lian,ZHU Qiang,WANG Na. Electrochemical Determination of Catechol with Nano-gold Modified Glassy Carbon Electrode[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(6): 468～471
张英,袁若,柴雅琴,傅英姿,卓颖,黎雪莲,朱强,王娜. 纳米金修饰玻碳电极测定邻苯二酚[J]. 理化检验－化学分册, 2007, 43(6): 468～471
被引情况:
【1】张立科,何琴,杨风岭,范顺利, "流动注射化学发光法测定痕量邻苯二酚",理化检验－化学分册 46, 897-899(2010)
【2】董永平,张超,张千峰, "邻苯二酚在金纳米粒子-碳纳米管复合修饰电极上的电化学行为",理化检验－化学分册 46, 1316-1318(2010)
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【3】Zhang S,Zhao H,John R.A dual-phase biosensing system for the determination of phenols in both aqueous and organic media[J].Anal Chim Acta,2001,441(1):95-105．
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【5】曲宁,崔九思,戚其平,等.反相高效液相色谱法快速同时测定苯代谢物的方法[J].中国卫生检验杂志,2001,11(6):661-663．
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【7】Rogers K R,Becker J Y,Cembrano J,et al.Viscosity and binder composition effects on tyrosinase-based carbon paste electrode for detection of phenol and catechol[J].Talanta,2001,54(6):1059-1065．
【8】Liu S Q,Yu J H,Ju H X.Renewable phenol biosensor based on a tyrosinase-colloidal gold modified carbon paste electrode[J].J Electroanal Chem,2003,540(2):61-67．
【9】何星存,李平,莫金恒.聚2,6-吡啶二甲酸膜对儿茶酚和对苯二酚电氧化的催化性能及其应用[J].化学研究与应用,2000,12(2):152-155．
【10】赵红,张玉忠,袁倬斌.聚5磺基水杨酸修饰电极上对苯二酚的电化学行为[J].分析试验室,2001,20(6):70-72．
【11】蔡火操,杜丹,孙亚楠,等.L-半胱氨酸自组装膜电极的表征及其对对苯二酚的差分脉冲伏安法测定[J].分析科学学报,2002,18(2):133-136．
【12】Finot M O,Braybrook G D,McDermott M T.Characterization of electrochemically deposited gold nanocrystals on glassy carbon electrodes [J].J Electroanal Chem,1999,466(2):234-241．
【13】Lu L P,Wang S O,Lin X Q.Fabrication of layer-by-layer deposited multiplayer films containing DNA and gold nanoparticle for norepinephrine biosensor[J].Anal Chim Acta,2004,519(1):161-166．
【14】Tang D P,Yuan R,Chai Y Q,et al.A novel immunosensor based on immobilization of hepatitis B surface antibody on platinum electrode modified colloidal gold and polyvinyl butyral as matrices via electrochemical impedance spectroscopy[J].Bioelectrochem,2004,65(1):15-22．
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