薄层色谱法分离紫杉醇和三尖杉宁碱
Separation of Taxol and Cephalomannine by Thin Layer Chromatography
摘 要
建立了用薄层色谱分离紫杉醇和三尖杉宁碱的方法.方法中以正己烷-二氯甲烷-甲醇-三乙胺(体积比4.5∶3.5∶0.5∶1.0)四元体系为展开剂,在硅胶薄层板上成功地分离了紫杉醇和三尖杉宁碱,所得斑点清晰、无拖尾.同时以该体系为展开剂,使用薄层色谱法指导分离提取紫杉醇和三尖杉宁碱过程中反相色谱流分的合并,方法快速、简单、成本低,取得了满意的结果.
紫杉醇
三尖杉宁碱
三乙胺
Thin layer chromatography
Taxol
Cephalomannine
Triethylamine
Abstract
In the method of separation of taxol and cephalomannine by thin layer chromatography,a solution containing n-hexane,dichloromethane,methanol and triethylamine (mixed in the ratio of 4.5∶3.5∶0.5∶1.0) was used as developing solvent (mobile phase) and the silica gel thin layer plate was used as the stationary phase.By using the modified mobile phase,taxol and cephalomannine were separated satisfactorily,the spots were separated clearly,sharply and without trailing.
中图分类号 O658.1
所属栏目 工作简报
基金项目
收稿日期 2005/12/11
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备注董慧茹(1945-),女,大连市人,教授,主要从事色谱和波谱的教学与研究工作.
引用该论文: DONG Hui-ru,LUO Li-na,ZHENG Yun,ZHAO Jing-cheng. Separation of Taxol and Cephalomannine by Thin Layer Chromatography[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(6): 472～475
董慧茹,雒丽娜,郑云,赵京城. 薄层色谱法分离紫杉醇和三尖杉宁碱[J]. 理化检验－化学分册, 2007, 43(6): 472～475
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参考文献
【1】Wani M C,Talyor H L,Monroe E W,et al.Plant antitumor agents.Ⅵ.Isolation and structure of taxol,a novel antileukemic and antitumor agent from Taxus brevifolia[J].J Am Chem Soc,1971,93(9):2325-2327．
【2】谈增毅.薄层色谱检识云南红豆杉化学成分的研究[J].基层中药杂志,1995,9(4):36-36．
【3】Kazimierz Glowniak,Grazyna Zgórka,Aleksandra Józefczyk,et al.Sample preparation for taxol and cephalomannine determination in various organs of Taxus SP[J].J Pharm Biomed Anal,1996,14:1215-1220．
【4】Shashi Bala,Uniyal G C,Chattopadhyay S K,et al.Analysis of taxol and major taxoids in Himalayan yew,Taxus wallichiana[J].J Chromatogra A,1999,858:239-244．
【5】Gwendolyn N C,Bruce D H,Susan B,et al.1H-and 13C-nmr Assignments for Taxol,7-epi-Taxol,and Cephalomannine[J].J Nat Prod,1992,55(4):414-423．
【2】谈增毅.薄层色谱检识云南红豆杉化学成分的研究[J].基层中药杂志,1995,9(4):36-36．
【3】Kazimierz Glowniak,Grazyna Zgórka,Aleksandra Józefczyk,et al.Sample preparation for taxol and cephalomannine determination in various organs of Taxus SP[J].J Pharm Biomed Anal,1996,14:1215-1220．
【4】Shashi Bala,Uniyal G C,Chattopadhyay S K,et al.Analysis of taxol and major taxoids in Himalayan yew,Taxus wallichiana[J].J Chromatogra A,1999,858:239-244．
【5】Gwendolyn N C,Bruce D H,Susan B,et al.1H-and 13C-nmr Assignments for Taxol,7-epi-Taxol,and Cephalomannine[J].J Nat Prod,1992,55(4):414-423．
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