流动注射化学发光法测定茶叶和蔬菜中亚硝酸根
FI-Chemiluminescence Determination of Nitrite in Tea and Vegetables
摘 要
利用亚硝酸根对鲁米诺-溴酸钾体系的化学发光具有显著增强作用,结合流动注射技术,建立了一种简单、快速测定亚硝酸根方法.方法的线性范围为4.0×10-3~4.0 mg·L-1;检出限为2.0×10-3mg·L-1(3σ);分别对 0.01 mg·L-1和0.40 mg·L-1的亚硝酸根进行了平行测定(n=11),其相对标准偏差分别为1.3%与1.5%.应用于茶叶、蔬菜中的亚硝酸根的测定,回收率为92.3%~108.0%.
流动注射
亚硝酸根
茶叶
蔬菜
Chemiluminescence
Flow-injection
Nitrite
Tea
Vegetable
Abstract
Based on the enhancement effect of NO-2 on the chemiluminescence (CL) reaction system of luminol and potassium bromate,and applying the flow injection (FI) technique,a new FI-CL method for determination of NO-2 was proposed.Linearity was kept in the range of 4.0×10-3-4.0 mg·L-1 of NO-2,with a detection limit (3σ) of 2×10-3mg·L-1.Precision was tested at the NO-2 concentration levels of 0.01 mg·L-1 and 0.40 mg·L-1,the values of RSD′s (n=11) obtained were 1.3% and 1.5% respectively.In the application of the proposed method to the determination of NO-2 in tea and vegetable samples,recoveries in the range of 92.3%-108.0% were obtained.
中图分类号 O657.31
所属栏目 工作简报
基金项目
收稿日期 2006/1/13
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备注王学锋(1963-),男,河南新乡人,副教授,硕士生导师,研究方向为环境分析.
引用该论文: WANG Xue-feng,SHI Xuan,PI Yun-qing. FI-Chemiluminescence Determination of Nitrite in Tea and Vegetables[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(6): 498～500
王学锋,史选,皮运清. 流动注射化学发光法测定茶叶和蔬菜中亚硝酸根[J]. 理化检验－化学分册, 2007, 43(6): 498～500
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参考文献
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【2】Cox R D,Frank C W.Determination of total N-nitroso content in cutting fluids[J].Anal Chem,1982,54(3):557．
【3】Nagashima K,Matsumoto M,Suzuki S.Determination of nitrate by using second-derivative spectrophotometry [J].Anal Chem,1985,57(11):2065．
【4】王耀光,齐秀贞,蔡金龙,等.基于偶联反应新极谱法测定痕量亚硝酸根的研究:Ⅱ.以苯酚为偶联试剂[J].分析化学,1994,22(8):767．
【5】Iskandarani Z,Pietrzyk D J.Zon interaction chromatography of organic anions in the presence of tetraalkylammonium salts[J].Anal Chem,1982,54(14):2601．
【6】Lu C,Qu F,Lin J M,et al.Flow-injection chemiluminescencent determination of nitrite in water based on the formation of per-oxynitrite from the reaction of nitrite and hydrogen peroxide[J].Anal Chim Acta,2002,474:107．
【7】杨维平,章竹君.流动注射化学发光抑制法测定痕量亚硝酸根的研究[J].陕西师范大学学报:自然科学版,1999,27(4):76．
【8】林金明.化学发光色谱柱后检测技术及其应用[J].色谱,2003,21(4):1．
【9】朱庆仁,孙登明.亚硝酸根的动力学分析法进展[J].岩矿测试,2000,19(3):199．
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