在近中性条件下,培氟沙星和Eu(Ⅲ)、EDTA 反应形成配合物,发生分子内能量转移,产生Eu(Ⅲ)的特征荧光(λem=615 nm,λex=278 nm),据此建立了灵敏、快速的检测培氟沙星的方法,方法线性范围为0.02~1.40 mg·L-1,检出限为0.02 mg·L-1,回收率为96.8%~100.7%.

It was reported that a ternary coordination complex was formed among Eu(Ⅲ),pefloxacin and EDTA,and due to the intramolecular energy transfer of the coordination complex,characteristic fluorescence spectra of Eu(Ⅲ) were observed at the peaks of 579 nm,592 nm and 615 nm,when excited at 278 nm. Based on these facts,a method for rapid determination of pefloxacin (PEFX) by fluorophotometry was proposed,using wavelengths of 278 nm and 615 nm as the excitation and emission wavelength respectively. Linearity range was found in the range of 0.02-1.40 mg of PEFX per liter,and detection limit obtained was 0.02 mg·L-1. Recovery tests were made,and the values of recovery obtained were in the range of 96.8%-100.7%.