非水介质毛细管电泳高频电导法测定姜黄中姜黄素
Determination of Curcumin in Curcuma by Capillary Electrophoresis with High Frequency Conductivity Detection in Nonaqueous Medium
摘 要
建立了药材姜黄中姜黄素含量的非水介质毛细管电泳高频电导测定方法.对非水介质体系和支持电解质的种类,浓度以及操作电压和进样时间等影响因素进行了优化.以无水甲醇作为分离介质,NH4OAc-HOAc为电解质,25.0 kV为分离电压,可在12 min内实现对姜黄素的分离检测.在最佳试验条件下,姜黄素的线性范围为3.0~140.0 mg·L-1,检出限为0.5 mg·L-1,回收率为95.3%~100.9%.
高频电导检测
姜黄素
姜黄
Capillary electrophoresis in nonaqueous medium
High-frequency conductivity detection
Curcumin
Curcuma
Abstract
It was reported in this paper that curcumine was separated from curcuma and determined by capillary electrophoresis with high-frequency conductivity detection in non-aqueous medium within 12 min. Optimum conditions for the analysis was described as follows: ① separation medium: anhydrous methanol;② electrolyte: 6 mmol·L-1 HOAc+8 mmol·L-1 NH4OAc solutions (pH 4.5);③ separation voltage: 25.0 kV. Linear relationship was kept between peak area and concentration of curcumine in the range from 3.0 to 140.0 mg per L of solution (r=0.997),with a detection limit of 0.5 mg·L-1. Samples of curcuma grown in Sichuan,Guangdong and Guangxi were collected and analyzed by the proposed method. Values of recovery test were in the range from 95.3%-100.9%.
中图分类号 O657.31
所属栏目 工作简报
基金项目 国家自然科学基金项目(20375049和20575080)
收稿日期 2006/1/6
修改稿日期
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备注徐健君(1979-),女,广东珠海人,硕士研究生,主要从事食品化工的检测分析工作.
引用该论文: XU Jian-jun,ZHAI Hai-yun,CHEN Zuan-guang,LIN Wei-feng,CAI Pei-xiang,MO Jin-yuan. Determination of Curcumin in Curcuma by Capillary Electrophoresis with High Frequency Conductivity Detection in Nonaqueous Medium[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(7): 588～590
徐健君,翟海云,陈缵光,林伟丰,蔡沛祥,莫金垣. 非水介质毛细管电泳高频电导法测定姜黄中姜黄素[J]. 理化检验－化学分册, 2007, 43(7): 588～590
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参考文献
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【3】周继斌,翁水旺.薄层扫描测定道地和异地引种姜黄中姜黄素含量[J].中国野生植物资源,2002,21(1):59-60．
【4】戚爱棣,于虹,朱晨.薄层扫描法测定姜黄、郁金、莪术中姜黄素的含量[J].天津中医学院学报,2002,21(2):32-33．
【5】戚爱棣.HPLC法测定姜黄、郁金、广西莪术中姜黄素的含量[J].中草药,2002,33(6):510-512．
【6】童荣生,席 庆,孙世明,等.反相高效液相色谱法测定七味姜黄胶囊中姜黄素的含量[J].中国医院药学杂志,2003,23(3):145-146．
【7】刘保启,胡孝忠,王玉春,等.高效毛细管电泳法测定姜黄中姜黄素类化合物[J].分析测试学报,2004,23(1):109-111．
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【9】Chen Z G,Mo J Y,Yang X Y,et al. A new capillary electrophoresis apparatus with piezoelectric ceramics high voltage source and amperometric detector[J]. Chin Chem Lett,1999,10(3):231-234．
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