流动注射抑制化学发光法测定盐酸环丙沙星
FI Inhibition-Chemiluminescence Determination of Ciprofloxacin Hydrochloride
摘 要
在碱性介质中,环丙沙星对鲁米诺-KIO4-Co2+反应体系产生的化学发光明显地受到抑制,且化学发光强度的抑制强度与环丙沙星的浓度成正比关系.基于上述现象,提出了环丙沙星的流动注射-化学发光测定法.环丙沙星的质量浓度在3.0×10-7~1.0×10-5g·L-1之间成线性关系,检出限为2.0×10-7g·L-1.对浓度水平为1.0×10-6g·L-1的盐酸环丙沙星进行11次平行测定,得到RSD(n=11)值为2.7%.应用此方法分析了环丙沙星药片,所得结果与按中国药典方法所得测定值保持一致,回收率试验的结果在98.5%~101.2%之间.
流动注射
抑制
盐酸环丙沙星
Chemiluminescence
Flow injection
Inhibition
Ciprofloxacin hydrochloride
Abstract
It was found that in an alkaline solution,chemiluminescence,produced by the reaction system of luminol-KIO4-Co2+,was remarkably inhibited by the presence of ciprofloxacin,and the magnitude of decrease of chemiluminescence intensity was found to be directly proportional to the concentration of ciprofloxacin.Based on these facts,a method of FI-chemiluminescence determination of ciprofloxacin was proposed.Linearity was kept in the range 3.0×10-7-1.0×10-5g·L-1 with a detection limit of 2.0×10-7g·L-1.Precision of the method was tested at the concentration level of 1.0×10-6g·L-1 and the RSD′s (n=11) found was 2.7%.The proposed was applied to the analysis of ciprofloxacin tablets,giving values of recovery in the range 98.5%-101.2%,and the analytical result obtained was in conformity with the result obtained by the method of chinese pharmacopoeia.
中图分类号 O657.31
所属栏目 工作简报
基金项目
收稿日期 2006/1/29
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备注唐玉海(1957-),男,江苏人,教授,研究方向为有机化学和药物分析.
引用该论文: TANG Yu-hai,YAO Hong,SUN Yuan-yuan. FI Inhibition-Chemiluminescence Determination of Ciprofloxacin Hydrochloride[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(8): 639～641
唐玉海,姚宏,孙媛媛. 流动注射抑制化学发光法测定盐酸环丙沙星[J]. 理化检验－化学分册, 2007, 43(8): 639～641
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参考文献
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【3】Katarzyna M,Genowefa P,Stefan T.Determination of ciprofloxacin and its impurities by capillary zone electrophoresis[J].J Chromatogr A,2004,1051(1-2):267-272．
【4】Du L,Xu Q,Yuan J.Fluorescence spectroscopy determination of fluoroquinolones by charge transfer reaction[J].J Pharm Biomed Anal,2003,33(4):693-698．
【5】Sutan S M,周执中.流动注射分光光度法测定制剂中的环丙沙星[J].国外医药抗生素分册,1994,15(3):175-178．
【6】胡娟,王宏.示波计时电位法测定环丙沙星含量[J].华西药学杂志,2002,17(3):213-214．
【7】狄俊伟,金民.盐酸环丙沙星的示波极谱测定及其机理研究[J].分析试验室,1995,14(4):33-36．
【8】梁耀东,李建中,章竹君.流动注射-化学发光测定盐酸环丙沙星[J].分析化学,1997,25(11):1307-1310．
【9】中华人民共和国卫生部药典委员会编.中华人民共和国药典二部[M].北京:化学工业出版社,2000:623．
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