Fluorospectrophotometric Determination of SudanⅠ by Its Fluorescence Quenching Effect on Human Serum Albumin
摘 要
研究了在0.1 mol·L-1 Tris-HCl缓冲溶液(pH 8.2)中,人血清白蛋白(HSA)的荧光性质以及由于苏丹红Ⅰ的存在而使HSA的荧光猝灭现象进行了研究.结果表明:当HSA溶液在280 nm波长激发时,其发射波长为337 nm.当有苏丹红Ⅰ存在时其荧光发射的强度将出现猝灭,而且荧光猝灭程度(ΔF)与苏丹红Ⅰ的质量浓度在0.5~7.5 mg·L-1(r=0.999 7)之间呈线性关系,方法的检出限(3Sb/k)为0.1 mg·L-1,测定限(10Sb/k)为0.5 mg·L-1,相对标准偏差(m=6,n=11)为0.7%~1.8%,加标回收率为91.9%~109.3%.
Abstract
The fluorescence characteristics of human serum albumin (HSA) and the fluorescence quenching by the presence of SudanⅠ were studied in 0.1 mol·L-1 Tris-HCl buffer medium of pH 8.2.It was found that emission fluorescence was observed at 337 nm (λem) when the HSA solution was excited at 290 nm (λex),and that the intensity of the emitted fluorescence was quenched by the addition of SudanⅠ solution.Linear relationship was kept between the magnitude of decrease in fluorescence intensity (ΔF) and the concentration of SudanⅠ in the range of 0.5-7.5 mg·L-1 (r=0.999 7).Detection limit (3Sb/k) and determination limit (10Sb/k) were found to be 0.1 mg·L-1 and 0.5 mg·L-1 respectively.Tests for precision and recovery at different concentration levels were made,giving values of RSD′s (m=6,n=11) and of recovery were found in ranges of 0.7%-1.8% and 91.9%-109.3% respectively..
中图分类号 O657.31
所属栏目 试验与研究
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收稿日期 2007/3/21
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备注杨胜圆(1975-),女,湖南吉首人,土家族,硕士,讲师,主要从事环境毒物检测研究方向.
引用该论文: YANG Sheng-yuan,Lv Chang-yin,FAN Xiang,LIU Yun-mei,XIANG Yan. Fluorospectrophotometric Determination of SudanⅠ by Its Fluorescence Quenching Effect on Human Serum Albumin[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(9): 713~715
杨胜圆,吕昌银,范翔,刘运美,向艳. 人血清白蛋白荧光猝灭法测定苏丹红Ⅰ[J]. 理化检验-化学分册, 2007, 43(9): 713~715
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参考文献
【1】Mazzetti M,Fascioli R,Mazzoncini I,et al.Determinationof 1-phenylazo-2-naphthol in chilli powder and in chillicontainingfood products by GPC clean-up and HPLC withLC/MS confirmation[J].Food Addit Contam,2004,21:935-941.
【2】Ming Ma,Xubiao Luo,Bo Chen,et al.Simultaneous determination of water soluble and fat soluble synthetic colorants in foodstuff by high performance liquid chromatography–diode array detection elect rospray mass spectrometry[J].Journal of Chromato-graphy A,2006,1103:170-176.
【3】吴惠勤,黄晓兰,黄芳.食品中苏丹红1号的GC-MS/SIM快速分析方法研究[J].分析测试学报,2005,24(3):1-5.
【4】王鲜俊,缪红,文君.薄层色谱法测定海椒面中苏丹红[J].中国卫生检验杂志,2005,5(12):1475-1476.
【5】余孔捷,杨方,卢声宇.分光光度法测定红辣椒及其产品中苏丹Ⅰ[J].中国卫生检验杂志,2004,4(5):596.
【6】易平贵,商志才,喻庆森.丝裂霉素C与牛血清白蛋白结合的作用研究[J].化学学报,2000,58(12):1649-1653.
【7】梁宏,宋仲容,周永洽,等.血清白蛋白的构象研究Ⅳ.pH诱导HSA构象变化的光谱研究[J].光谱学与光谱分析,1994,14(6):39-42.
【2】Ming Ma,Xubiao Luo,Bo Chen,et al.Simultaneous determination of water soluble and fat soluble synthetic colorants in foodstuff by high performance liquid chromatography–diode array detection elect rospray mass spectrometry[J].Journal of Chromato-graphy A,2006,1103:170-176.
【3】吴惠勤,黄晓兰,黄芳.食品中苏丹红1号的GC-MS/SIM快速分析方法研究[J].分析测试学报,2005,24(3):1-5.
【4】王鲜俊,缪红,文君.薄层色谱法测定海椒面中苏丹红[J].中国卫生检验杂志,2005,5(12):1475-1476.
【5】余孔捷,杨方,卢声宇.分光光度法测定红辣椒及其产品中苏丹Ⅰ[J].中国卫生检验杂志,2004,4(5):596.
【6】易平贵,商志才,喻庆森.丝裂霉素C与牛血清白蛋白结合的作用研究[J].化学学报,2000,58(12):1649-1653.
【7】梁宏,宋仲容,周永洽,等.血清白蛋白的构象研究Ⅳ.pH诱导HSA构象变化的光谱研究[J].光谱学与光谱分析,1994,14(6):39-42.
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