磷钼蓝光度法测定酸性甘油溶液中1,3-二羟基丙酮
Determination of 1,3-Dihydroxyacetone in Acidic Glycerol Solution by Molybdo-Phosphorus Blue-Photometry
摘 要
提出了酸性甘油溶液中1,3-二羟基丙酮(DHA)的光度测定的方法.简述了光度法测定DHA的原理,讨论了影响DHA测定的因素和规律.质量浓度在0.60 g·L-1以内,煮沸时间15 min,显色剂用量为2 mL,吸光度与质量浓度呈线性关系.校准曲线回归方程为Y=2.308 X-0.025 9,r=0.995 8,共存的甘油醛、甘油酸、羟基丙酮酸等物质对DHA的测定基本无干扰.
磷钼酸试剂
磷钼蓝
1,3-二羟基丙酮(DHA)
酸性甘油溶液
Photometry
Molybdo-phosphate color reagent
Molybdo-phosphorus blue
1,3-Dihydroxyacetone
Acidic glycerol solution1,3-
Abstract
The molybdo-phosphorus blue-photometry was applied to the determination of 1,3-dihydroxyacetone (DHA) in acidic glycerol solution.Based on a systematic study on the conditions of the color reaction,the amount of the color reagent (a mixed solution of molybdic acid and phosphoric acid) used was 2.0 mL per 50 mL of reaction solution,and the reaction mixture was heated for 15 min in a water bath at 100 ℃.Beer′s law was obeyed up to 0.60 g of DHA per liter of solution,corresponding to the linear regression equation Y=2.308 X-0.025 9 (r=0.995 8).DHA itself acted as a reductant for the molybdo-phosphate in the color reaction.The presence of small amounts of GALD,GLYA,HYPA and etc in the sample solution showed no significant interferences to the determination of DHA.
中图分类号 O657.31
所属栏目 工作简报
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收稿日期 2007/1/13
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备注王敬锋(1977-),男,湖北襄樊人,硕士研究生,主要从事有机催化与合成的研究.
引用该论文: WANG Jing-feng,CHEN Xian-ming,ZHAO Fan. Determination of 1,3-Dihydroxyacetone in Acidic Glycerol Solution by Molybdo-Phosphorus Blue-Photometry[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(9): 764～765
王敬锋,陈先明,赵帆. 磷钼蓝光度法测定酸性甘油溶液中1,3-二羟基丙酮[J]. 理化检验－化学分册, 2007, 43(9): 764～765
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参考文献
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【2】Fedoroko M.Determination of DL-glyceraldehyde,dihydroxyacetone,and methylglyoxal in a mixture [J].J Electroanal Chem,1967,14(3):356-367．
【3】GB 6324.5-1986羰基化合物含量的测定容量法[S]．
【4】Fedoronko M,Fuleova E,Danieliszym W.Estimation of DL-glyceraldhyde,dihydroxyacetone,methylglyoxal by oxidation with sodium periodate[J].Chem Zvesli,1973,27(1):67-73．
【5】郑裕国,张 霞,沈寅初.微生物转化甘油生产1,3-二羟基丙酮的菌株筛选 [J].浙江工业大学学报,2001,29(2):124-127．
【6】杭州大学化学系分析化学教研室编.分析化学手册:第三分册[M].2版.北京:化学工业出版社,1997:524．
【7】Walter R Campbell.The quantitive determination of dihydroxyacetone[J].J Biol Chem,1926,67(1):59-68．
【8】Otto Folin,Hsien Wu.A system of analysis [J].J Biol Chem,1920,41(3):367-374．
【9】Garcia R,Besson M,Gallezot P.Chemo selective catalytic oxidation of glycerol with air on platinum metals[J].Applied Catalysis A:General,1995,127(2):165-176．
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