应用荧光纳米颗粒测定痕量铜
Determination of Trace Amounts of Copper with Fluorescent Nanoparticles
摘 要
制备了异硫氰酸罗丹明B(TRITC)荧光纳米颗粒,于pH 6.0的磷酸盐缓冲溶液中此荧光纳米颗粒在558 nm光的激发下,于586 nm波长处发射荧光.铜(Ⅱ)的存在可使此荧光纳米颗粒溶液的荧光发生猝灭.借此,构建了一种检测微量铜的方法.在最佳试验条件下,该方法测定铜的线性范围为5.00×10-5~3.50×10-4mol·L-1,其回归方程为F/F0=8.425 2-1.009×10-6c,检出限为3.55×10-5mol·L-1.
荧光纳米颗粒
荧光猝灭效应
铜离子
Fluorospectrophotometry
Fluorescent nanoparticles
Fluorescence quenching effect
Cupric ion
Abstract
Isothiocyanato-rhodamine B doped fluorescent nanoparticles were prepared and the fluorescence characteristics were studied.As shown by experimental result,fluorescence emission at 586 nm was observed when aqueous solution of the fluorescent nanoparticles was excited at 558 nm.And the emitted fluorescence was found to be quenched by cupric ion,in a phosphate buffer solution of pH 6.0.Based on these facts,a fluorospectrophotometric method for the determination of traces of copper was proposed.Linear relationship was kept in the range of 5.00×10-5-3.50×10-4mol·L-1 Cu(Ⅱ),according to the linear regression equation F/F0=8.425 2-1.009×10-6c.Detection limit (3S) was found to be 3.55×10-5mol·L-1.
中图分类号 O657.31
所属栏目 试验与研究
基金项目 上海市教委项目(05DZ01、05JG05054、06ZZ16)和化学生物传感与计量学国家重点实验室课题(2005001)
收稿日期 2007/3/14
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备注李瑞娜(1982-),女,河南濮阳人,硕士,主要研究方向为纳米生物技术.
引用该论文: LI Rui-na,HUANG Shan-sheng,QU Yong-xia,SHEN Jian,JIN Li-xia. Determination of Trace Amounts of Copper with Fluorescent Nanoparticles[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(10): 805～807
李瑞娜,黄杉生,屈永霞,沈健,金丽霞. 应用荧光纳米颗粒测定痕量铜[J]. 理化检验－化学分册, 2007, 43(10): 805～807
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