DNA与5-Br-PAN-S分子间结合反应
Combination Reaction Between Molecules of DNA and 5-Br-PAN-S
摘 要
利用光谱修正技术研究脱氧核糖核酸(DNA)与5-Br-PAN-S(L)分子间的相互作用,通过pH 8.04的介质中DNA与5-Br-PAN-S反应的光谱研究,测定了结合产物的物理化学参数:10 ℃时探针L与DNA的结合比为4.3,结合常数K=0.6118×106;30 ℃时探针L与DNA的结合比为3.7,结合常数K=0.676 9×106.此结合反应符合Langmuir吸附方程,该方法可应用于痕量DNA的测定.
Langmuir吸附
DNA
5-Br-PAN-S
Spectral correction technique
Langmuir adsorption
DNA
5-Br-PAN-S
Abstract
The interaction of molecules of DNA with 5-Br-PAN-S was studied by applying the spectral correction technique.Through absorptio-spectrometry,In a buffer medium of pH 8.04,the physical chemical parameters of the combination product were studied by spectro-photometry.The combination ratio between 5-Br-PAN-S and DNA (L∶DNA) at 10 ℃ was found to be 4.3,with combination constant of 0.611 8×106;while at 30 ℃,the ratio was 3.7 with combination constant of 0.676 9×106.It was found that the interaction conformed with the Langmuir adsorption equation.This interaction was feasible to be used for the determination of DNA.
中图分类号 O65
所属栏目 试验与研究
基金项目 国家自然科学基金(20477030);上海市重点基础研究项目(04JC14072)
收稿日期 2007/1/12
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备注潘碌亭(1964-),男,安徽人,副教授,主要从事环境工程与环境科学专业的教学与科研工作.
引用该论文: PAN Lu-ting,LI Lu,GAO Hong-wen,LUO Hua-fei. Combination Reaction Between Molecules of DNA and 5-Br-PAN-S[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(10): 808～810
潘碌亭,李璐,郜洪文,罗华飞. DNA与5-Br-PAN-S分子间结合反应[J]. 理化检验－化学分册, 2007, 43(10): 808～810
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参考文献
【1】刘蓉,齐震,朱小蓬,等.真核生物DNA非编码区的组分分析[J].生物化学与生物物理进展,2002,29(2):256-258．
【2】曹瑛,李一峻,何锡文.中性红作为DNA作用方式光谱探针的研究[J].高等学校化学学报,1999,19(5):709-712．
【3】Zhao Y,Wang D Y,Guo X Q,et al.Determination of DNA and RNA by their quenching effect on the fluorescence of the Tb3+-Tiron complex[J].Anal Chim Acta,1997,353:329-335．
【4】朱永春,程广金.圆二色谱电化学研究进展[J].自然科学进展,2000,12(10):1072-1077．
【5】GAO H W,Jiang Jun,Yu Lin-Qian.Study on spectrometric probe of protein solution with p-iodochlorophosphonazo as adsorbate by MPASC technique[J].Analyst,2001,126(4):528-533．
【6】陈建荣,吴小华,蒋伟刚.用5-Br-PAN-S作络合滴定剂测定铜[J].理化检验-化学分册,1998,15(3):466-467．
【7】郜洪文,李玉成,江俊.探针体耐尔蓝(NB)与DNA结合反应研究[J].化学物理学报,2001,14(6):737-743．
【8】王红艳,张胜义,郜洪文.DNA-夜蓝结合光谱研究[J].安徽大学学报:自然科学版,2003,27(3):77-80．
【9】Huang C Z,Li Y F,Mao J G,et al.Determination of protein concentration bu enhacement of the preresonance light-scattering of α,β,γ,δ-tetrakis (5-sulfothionyl) porphine[J].Analyst,1998,123(6):1401-1406．
【2】曹瑛,李一峻,何锡文.中性红作为DNA作用方式光谱探针的研究[J].高等学校化学学报,1999,19(5):709-712．
【3】Zhao Y,Wang D Y,Guo X Q,et al.Determination of DNA and RNA by their quenching effect on the fluorescence of the Tb3+-Tiron complex[J].Anal Chim Acta,1997,353:329-335．
【4】朱永春,程广金.圆二色谱电化学研究进展[J].自然科学进展,2000,12(10):1072-1077．
【5】GAO H W,Jiang Jun,Yu Lin-Qian.Study on spectrometric probe of protein solution with p-iodochlorophosphonazo as adsorbate by MPASC technique[J].Analyst,2001,126(4):528-533．
【6】陈建荣,吴小华,蒋伟刚.用5-Br-PAN-S作络合滴定剂测定铜[J].理化检验-化学分册,1998,15(3):466-467．
【7】郜洪文,李玉成,江俊.探针体耐尔蓝(NB)与DNA结合反应研究[J].化学物理学报,2001,14(6):737-743．
【8】王红艳,张胜义,郜洪文.DNA-夜蓝结合光谱研究[J].安徽大学学报:自然科学版,2003,27(3):77-80．
【9】Huang C Z,Li Y F,Mao J G,et al.Determination of protein concentration bu enhacement of the preresonance light-scattering of α,β,γ,δ-tetrakis (5-sulfothionyl) porphine[J].Analyst,1998,123(6):1401-1406．
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