碱性二苯基萘基甲烷染料褪色分光光度法测定藻酸钠
Spectrophotometric Determination of Sodium Alginate by Decoloration of Some Basic Dyes
摘 要
在B-R缓冲溶液中,藻酸钠与维多利亚蓝4R、维多利亚蓝B、夜蓝等碱性二苯基萘基甲烷染料发生反应,形成结合产物,染料发生明显的褪色.对于不同染料,最大褪色波长分别位于612 nm(维多利亚蓝4R体系)、616 nm(维多利亚蓝B体系)、614 nm(夜蓝体系).体系吸光度的降低与藻酸钠的质量浓度成正比.藻酸钠的质量浓度在5.0 mg·L-1(维多利亚蓝4R体系)、3.0 mg·L-1(维多利亚蓝B体系)、3.0 mg·L-1(夜蓝体系)以内符合比耳定律,摩尔吸光率根据染料的不同在4.8×106~9.5×106L·mol-1·cm-1之间,其中以维多利亚蓝4R体系最为灵敏,对藻酸钠的检出限为21 μg·L-1.以维多利亚蓝4R体系为例研究共存物质的影响,表明方法选择性好.此法用于面条样品中藻酸钠的分析测定,结果满意.
藻酸钠
碱性染料
Spectrophotometry
Sodium alginate
Basic dyes
Abstract
In a BR-buffer of pH 3.0,association compounds were formed between large anion of sodium alginate (NaAGN) and cation R+ of some basic dyes,e.g.victoria blue 4R (VB4R),victoria blue B (VBB) and night blue (NB),leading to color-fading of the dye reagents.Linear relationships between the magnitude of color-fading intensity (ΔA) and concentration of NaAGN were obtained in the ranges within 5.0 mg·L-1 (for VB4R system) and 3.0 mg·L-1 (for either VBB or NB system).Absorbance were measured at the absorption maxima 612 nm,616 nm and 614 nm for VB4R,VBB and NB respectively.Values of molar absorptivity for the 3 dyes were found in the range 4.8×106-9.5×106L·mol-1·cm-1,among which the value of VB4R was the highest.Detection limit of this method with VB4R as reactant was 21 μg·L-1.Selectivity was tested giving satisfactory results for determination of NaAGN.In applying the method to analysis of noodle sample,average values of RSD (n=7) and recovery obtained were 2% and 105% respectively.
中图分类号 O657.31
所属栏目 试验与研究
基金项目 国家自然科学基金资助项目(No.20475045)
收稿日期 2006/10/14
修改稿日期
网络出版日期
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备注王祥洪(1978-),男,重庆市人,重庆长江师范学院讲师,硕士研究生,主要从事分子光谱研究.
引用该论文: WANG Xiang-hong,YANG Ji-dong,LIU Shao-pu. Spectrophotometric Determination of Sodium Alginate by Decoloration of Some Basic Dyes[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(10): 811～814
王祥洪,杨季冬,刘绍璞. 碱性二苯基萘基甲烷染料褪色分光光度法测定藻酸钠[J]. 理化检验－化学分册, 2007, 43(10): 811～814
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【3】Kawasaki Y,Ishiwata H,Yamada T.Determination method for sodium alginate in foods[J].Shokuhin Eiseigaku Zasshi,1998,39(5):297-302．
【4】Pfenninger H B,Anderegg P,Vykoukal E.Alginate determination in beer[J].J Am Soc Brew Chem,1983,41(1):40-42．
【5】Sagalovich V P,Kulikov Y M,Mushnikova O L,et al.Quantitative determination of sodium alginate[J].Otkrytiya Izobret,1991(31):182．
【6】Pechanek U,Blaicher G,Pfannhauser,et al.Electrophoretic method for qualitative and quantitative analysis of gelling and thicking agents[J].J Assoc Off Anal Chem,1982,65(3):745-752．
【7】Padmoyo M,Miserez A.Identification of gelling and thickening agents permitted in Switzerland by electrophoresis and staining on cellulose acetate strips[J].Mitt Geb Lebensmittelunters Hyg,1967,58(1):31-49．
【8】Better B,Amado R,Neukom H.Electrophoretic determination of gelling and thicking agents on silylated glass fiber paper[J].Mitt Geb Lebensmittelunters Hyg,1985,76(1):69-82．
【9】Sjoberg A M,Pyysalo H.Indentification of food thickeners by monitoring of their pyrolytic products[J].J Chromatogr,1985,319(1):90-98．
【10】Scherz H.Analysis of thickening agents in foods Lebensmittelchem[J].Greichtl Chem,1985,39(2):32-33．
【11】Cantafora A,DiMuccio A,Villalohos D A.Chromatography of thicking agents in some foods[J].Riv Soc Ital Sci Aliment,1974,3(4):99-102．
【12】Toyoda M,Yomota C,Ito Y,et al.High performance liquid chromatographic method for the determination of sodium alginate in foods[J].Shokuhin Eiseigaku Zasshi,1985,26(2):189-194．
【13】Rourke T J,Mandigo R W.HPLC quantitation of alginate or pectin added to lean ground pork[J].J Food Sci,1993,58(5):973-977．
【14】Kawasaki Y,Ishiwata H,Yamada T.Determination method for sodium alginate in foods[J].Shokuhin Eiseigaku Zasshi,1998,39(5):297-302．
【15】Kennedy J F,Bradshaw I J.The rapid quantitative determination of alginates by poly (hexomethylenebiguanrdinium ohloride) complexation in industrial liquors extracted from brown seaweed[J].Carbohydr Polym,1987,7(1):35-50．
【16】Murata Y,Kawashima S,Miyamoto E.Colorimetric determination of alginates and fragments thereof as the 2-pitrophenylhydrazide[J].Carbohydr Res,1990,208:289-292．
【17】Kennedy J F,Bradshaw I J.A rapid method for the assay of alginates insolution using polyhexamethylenebiguanidinium chloride[J].Br Polym J,1984,16(2):95-101．
【18】日本厚生省环境卫生及食品化学课.食品中添加剂分析方法[M].马尔骧,高鹤娟,戴 莹,等译.北京:中国标准出版社,1988:568-574．《分析试验室》2008年征订启事《分析试验室》是中文核心期刊,月刊,大16开,128页,国内外公开发行,每月1日出版.
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