线性扫描伏安法测定人血清白蛋白
Linear Sweeping Voltammetric Determination of Human Serum Albumin and Its Analytical Application
摘 要
建立了一种测定人血清白蛋白的新方法.应用线性扫描伏安法研究了灿烂甲酚蓝(BCB)与人血清白蛋白(HSA)的相互作用的条件,当BCB与HSA作用后,其峰电流下降且峰电位负移,峰电流的下降值的二阶导数(ΔI″p)同HSA的质量浓度在2.0~60.0 mg·L-1范围内呈线性关系,相关系数为0.997 0.方法的检出限为2.0 mg·L-1，用于健康人血清样品的测定,回收率在95.6%~107.5%之间.
灿烂甲酚蓝
人血清白蛋白
临床检验
Linear sweeping voltammetry
Human serum albumin
Brilliant cresol blue
Clinical testing
Abstract
Linear sweeping voltammetry was applied to the determination of human serum albumin (HSA) bese on its interaction with brillianl cresol blue (BCB).Conditions for the reaction were studied and optimized.In a B-R buffer medium of pH 6.5,reductive peak of BCB was observed at -0.1 V (vs.SCE),and in the presence of HSA,due to their interaction,the reductive peak was shifted negatively with decrease in peak current.Linear relationship between 2nd derivative of the magnitude of decrease in peak current (ΔI″p) and the concentration of HSA was found in the range of 2.0-60.0 mg·L-1 (r=0.997 0).The detection limit was found to be 2.0 mg·L-1.Test for recovery was made by standard addition method and the results obtained were in the range of 95.6%-107.5%.
中图分类号 O657.31
所属栏目 工作简报
基金项目 国家自然科学基金(20075013)资助项目
收稿日期 2006/8/25
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备注王学亮(1977-),男,山东东明人,博士研究生,主要从事电分析化学研究.
引用该论文: WANG Xue-liang,JIAO Kui. Linear Sweeping Voltammetric Determination of Human Serum Albumin and Its Analytical Application[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(11): 940～942
王学亮,焦奎. 线性扫描伏安法测定人血清白蛋白[J]. 理化检验－化学分册, 2007, 43(11): 940～942
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参考文献
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【6】高峰,朱昌青,汪乐余,等.灿烂甲酚蓝与表面活性剂的作用及其在蛋白质测定中的应用[J].分析化学,2002,30(3):324．
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【8】郑石英,周春,罗登柏.刚果红与血清白蛋白相互作用的极谱分析[J].分析测试学报,2003,22(4):64．
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