将含磷脂酰肌醇的大豆磷脂水解后,进行乙酰衍生化得到肌醇六乙酯,然后用气相色谱法测定肌醇六乙酯,从而计算出大豆磷脂中磷脂酰肌醇的含量.对一大豆磷脂试样,按提出方法测定6次,得磷脂酰肌醇含量的平均值为8.61 mg·g-1,测定结果的相对标准偏差为2.2%.又在此试样的基础上加入不同量的磷脂酰肌醇标准(10.0~110.0 mg)作回收试验,所得回收率结果为95.6%~104.2%.

Through hydrolysis of soy lecityin,the phosphatidyl inositol produced was acetylated with acetyl anhydride to give inositol hexacetate,which was then determined by gas chromatography.Amount of phosphatidyl inositol contained in the sample was calculated indirectly from the amount of inositol hexacetate found by GC.Six portions of a soy lecithin sample were taken and its phosphatidyl inositol content were determined by the proposed method,giving an average value of 8.61 mg·g-1 with a value of RSD (n=6) 2.2%.Test for recovery was made by adding different amounts of standard phosphatidyl inositol from 10.0 mg to 110.0 mg,values of recovery found were in the range of 95.6%-104.2%.