应用发光细菌法检测了经次氯酸钠处理的压载水的生物毒性,制备了模拟海水并用于试验中.在模拟压载水样中加入人工孵化的卤虫作为水生物活体,用明亮发光杆菌T3小种作为检测用的发光菌.取压载水样多份,分别用不同有效氯浓度(5,10,20 mg·L-1)的次氯酸钠处理不同的时间(2,6,16,24 h).在处理过程中每隔6 h检测其有效氯,要求始终保持有效氯在设定的浓度.处理结束后,用滤网除去水中的残留虫体,并加入硫代硫酸钠使残余氯还原.定量分取此溶液,按国家标准GB/T 15441-1995中所述方法测定其发光强度.试验结果表明:当用有效氯浓度为10 mg·L-1的次氯酸钠,经6 h以内的时间处理的压载水具有最低的生物毒性.

Luminescent bacteria test was applied to the detection of biological toxicity of hypochlorite treated ballast water.Simulated sea water was prepared and used in the experiments.Artificially incubated aquatic insect (the “brine insect”) was added in the ballast water as living organisms and photobacterium phosphoreum T3 spp was used as the luminescent bacteria in the detection.Samples of ballast water ware treated individually with hypochlorite having different concentration of available chlorine (5,10,20 mg·L-1) and for different time periods (2,6,16 and 24 h),during which the available chlorine was determined every 6 h interval and its concentration kept at the prescribed values.At the end of treatment,remnant of the insect was removed by mesh filter,and the remaining chlorine was rendered inactive by reduction with sodium thiosulfate.An aliquat of the solution was taken and its luminescence intensity were determined by the standard method in GB/T 15441-1995.As shown by experimental results,low biological toxicity was obtained when the hypochlorite treatment was performed with 10 mg·L-1 of available chlorine and for a period of less than 6 h.