考察了红霉素与甲基紫电荷转移反应的条件.试验结果表明:以水为溶剂,红霉素与甲基紫在50 ℃水浴中进行电荷转移反应生成1∶1稳定的配合物,其最大吸收波长为583 nm.摩尔吸光率为1.61×104L·mol-1·cm-1,对红霉素标准进行9次测定,相对标准偏差为0.91%.红霉素的质量浓度在2.1 mg/25 mL以内服从比耳定律,方法的检出限(S/N=3)为1.51 mg·L-1,回收率大于97.5%,方法可用于红霉素肠溶片和兽用红霉素测定.

It was reported that in a solution of pH 1.0,through the charge transfer reaction,a stable coordination complex with mole ratio of 1 to 1 was formed between erthromycin and methyl violet,by heating at 50 ℃ for 10 min in a water bath.The absorption maxima of the complex was found at the wavelength of 583 nm,where the molar absorptivity of 1.61×104L·mol-1·cm-1 was obtained.Beer′s law was kept up to the concentration of 2.10 mg of erythromycin per 25 mL of solution.Precision was tested at the concentration level of 0.52 mg/25 mL,and the value of RSD (n=9) obtained was 0.91%.Detection limit (S/N=3) of the method was found to be 1.51 mg·L-1.Recovery test was made by standard addition method,values obtained were ≥97.5%.