HPLC Determination of Diethylstibestrol in Chicken
摘 要
采用高效液相色谱法(HPLC)测定鸡肉中己烯雌酚残留量,将碎鸡肉用甲醇超声提取,合并提取液浓缩近干,用甲醇溶解残渣,所得溶液用于HPLC测定.Symmetry C18用作色谱柱,甲醇与0.012 8 mol·L-1磷酸二氢钠溶液以63比37(体积比)混合后作为流动相,流速为1.0 mL·min-1.在230 nm波长处,用二极管阵列检测器检测.对鸡肉样品作了分析,测定结果的相对标准偏差(n=5)均小于10%,回收试验给出回收率在87.5%~92.3%之间.测得方法的检出限(S/N=3)为20 μg·kg-1.
Abstract
High performance liquid chromatography (HPLC) was applied to the determination of residual diethylstibestrol in chicken as food. Smashed and homogenized chicken sample was extracted ultrasonically with methanol for several times as directed in the procedure. The extracts were combined and evaporated to near dryness. The residue was taken up with 1 mL of methanol and the solution was used for HPLC determination. Symmetry C18 was used as the chromatographic column and a mixture of methanol and 0.012 8 mol·L-1 NaH2PO4 solution mixed in the ratio of 63 to 37 ( by volume),adjusted to pH 5,was used as the mobile phase at a flow-rate of 1.0 mL·min-1. UV-detection at 230 nm with array photodiode was used in the determination. Detection limit (S/N=3) was found to be 20 μg·kg-1. Sample of chicken was analyzed by this method and values of RSD′s (n=5) and recovery were found to be less than 10% and in the range of 87.5% to 92.3% respectively.
中图分类号 O657.37
所属栏目 工作简报
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收稿日期 2007/7/19
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备注程盛华(1980-),男,江西九江人,研究实习员,主要研究方向为兽药残留分析.
引用该论文: CHENG Sheng-hua,YANG Chun-liang,ZHA Yu-bing,HUANG Mao-fang. HPLC Determination of Diethylstibestrol in Chicken[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(1): 15~16
程盛华,杨春亮,查玉兵,黄茂芳. 高效液相色谱法测定鸡肉中己烯雌酚[J]. 理化检验-化学分册, 2008, 44(1): 15~16
被引情况:
【1】杜凌云,朱琳,王术皓, "荧光光度法测定己烯雌酚",理化检验-化学分册 49, 985-987(2013)
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参考文献
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【4】GB/T 5009.108-2003畜禽肉中己烯雌酚的测定[S].
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