高效液相色谱法与酶免疫法快速测定鲫鱼中残留显/隐色孔雀石绿的比较
Comparison of Rapid Determination of Residual Malachite Green in Crucian by HPLC and Enzyme Immunoassay
摘 要
采用高效液相色谱(HPLC)法与酶免疫法(EIA)两种方法检测鲫鱼中残留显/隐色孔雀石绿的含量,对这两种分析方法所得的回收率进行了比较 ,当添加浓度在5.0~50.0 μg·kg-1时,其中HPLC法所测得的回收率为68.2%~90.0%,相对标准偏差小于7.0%,检出限为2 μg·kg-1;EIA法所测得的回收率为72.4%~92.3%,相对标准偏差小于6.8%,检出限为1 μg·kg-1;两种方法均具有高效、快速、稳定的特点,能满足日常检验工作的需要.
酶免疫法
鲫鱼
显/隐色孔雀石绿
鲫鱼
HPLC
Enzyme immunoassay
Malachite green
Leucomalachite green
Crucian
Abstract
High performance liquid chromatography (HPLC) and enzyme immunoassay (EIA) were separately applied to the rapid determination of residual malachite green (MG) and leucomalachite green (LMG) in crucian samples. Values of recovery of both methods found were compared. When the standard of MG and LMG were added in the range from 5.0-50.0 μg·kg-1 to crucian samples,the mean values of recoveries of HPLC determinations ranged from 68.2% to 90.0% with RSD′s (n=7) less than 7.0%,and detection limit of 2 μg·kg-1;while the mean values of recoveries of EIA determinations ranged from 72.4% to 92.3%,with RSD′s (n=7) less than 7.0%,and detection limit of 1 μg·kg-1. Both of the methods were characterized by their high efficiency,rapidity and stability,and were feasible to be used in the daily routine analysis.
中图分类号 O657.37
所属栏目 工作简报
基金项目
收稿日期 2007/1/8
修改稿日期
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备注陈其勇(1978-),男,山东泰安人,硕士,工程师,主要从事农兽药及食品添加剂的残留检测研究.
引用该论文: CHEN Qi-yong,GE Bao-kun,MI Jie-bo,LIU Xuan,WANG Yun-feng,ZHOU Rong. Comparison of Rapid Determination of Residual Malachite Green in Crucian by HPLC and Enzyme Immunoassay[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(1): 68～70
陈其勇,葛宝坤,宓捷波,刘煊,王云凤,周荣. 高效液相色谱法与酶免疫法快速测定鲫鱼中残留显/隐色孔雀石绿的比较[J]. 理化检验－化学分册, 2008, 44(1): 68～70
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参考文献
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【3】GB/T 19857-2005水产品中孔雀石绿和结晶紫残留量的测定[S]．
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【6】郭德华,叶长淋,李波,等.高效液相色谱-质谱法测定水产品中孔雀石绿及代谢产物[J].分析测试学报,2004,23(增刊):206-208．
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【2】Culp S J,Blankenship L R,Kusewitt D F,et al. Toxicity and metabolism of malachite green and leucomalachite green during short-term feeding to Fischer 344 rats and B6C3F1 mice[J]. Chemico-Biological interactions,1999,122(3):153-170．
【3】GB/T 19857-2005水产品中孔雀石绿和结晶紫残留量的测定[S]．
【4】Roybal J E,Denver C O,Pfenning A P,et al. Robert K Munns,et al. Determination of malachite green and its metabolite leucomalachite green,in catfish tissue by HPLC with visible detection[J]. Journal of AOAC International,1995,78:453-457．
【5】葛宝坤,王云凤,常春艳,等.固相萃取-液相色谱法快速测定水产品中的孔雀石绿、结晶紫及其代谢物[J].中国卫生检验杂志,2006,16(1):45-47．
【6】郭德华,叶长淋,李波,等.高效液相色谱-质谱法测定水产品中孔雀石绿及代谢产物[J].分析测试学报,2004,23(增刊):206-208．
【7】Plakas S M,El Said K R. Optimization liquid chromatographic method for determination of malachite green and its metabolites in fish tissues[J]. Journal of AOAC International,1995,78(6):1388-1394．
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