高效毛细管电泳-电化学检测滴鼻液中盐酸麻黄碱和磺胺嘧啶
Determination of Ephedrine Hydrochloride and Sulfadiazine in the Nasal Drops by Capillary Electrophoresis with Electrochemical Detection
摘 要
采用毛细管电泳-电化学检测法(CE-ED)同时测定滴鼻液中盐酸麻黄碱和磺胺嘧啶的含量,考察了缓冲液酸度和浓度、氧化电位、分离电压和进样时间等试验参数对分离检测的影响.在最佳试验条件下,工作电极为直径300 μm的碳圆盘电极,检测电位为0.95 V(vs.SCE),缓冲液为50 mmol·L-1硼砂-硼酸溶液(pH 8.0),分离电压为17 kV,上述两组分在7 min内即可实现分离.盐酸麻黄碱和磺胺嘧啶在三个数量级的范围内呈线性,检出限(S/N=3)分别为7.0×10-5g·L-1和3.0×10-5g·L-1,回收率在97.0%~98.0%之间.
电化学检测
盐酸麻黄碱
磺胺嘧啶
滴鼻液
Capillary electrophoresis
Electrochemical detection
Ephedrine Hydrochloride
Sulfadiazine
Nasal drops
Abstract
A method based on capillary electrophoresis with electrochemical detection (CE-ED) was proposed for the determination of ephedrine hydrochloride and sulfadiazine in nasal drops. The effects of working electrode potential,pH and concentration of moving buffer,separation voltage and time of sample introduction on CE-ED were studied. Under the optimum conditions,the analytes could be separated in a 50 mmol·L-1 borate buffer (pH 8.0) within 7 min. A 300 μm diameter carbon disk electrode showed good response at 0.95 V (vs.SCE) for all analytes. The response was linear over three orders of magnitude with detection limits (S/N=3) ranging from 7.0×10-5g·L-1 to 3.0×10-5g·L-1 for the analytes. The method has been successfully applied for the analysis of substantial sample,with values of recovery in the range of 97.0%-98.0%.
中图分类号 O657.31
所属栏目 工作简报
基金项目
收稿日期 2007/6/9
修改稿日期
网络出版日期
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备注唐祝兴(1974-),男,辽宁人,博士研究生,研究方向为毛细管电泳.
引用该论文: TANG Zhu-xing,ZENG Yi-kun,ZHANG Wei-dong,ZHOU Yun,ZANG Shu-liang,HE Pin-gang,FANG Yu-zhi. Determination of Ephedrine Hydrochloride and Sulfadiazine in the Nasal Drops by Capillary Electrophoresis with Electrochemical Detection[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(1): 71～73
唐祝兴,曾祎琨,张卫东,周韵,臧树良,何品刚,方禹之. 高效毛细管电泳-电化学检测滴鼻液中盐酸麻黄碱和磺胺嘧啶[J]. 理化检验－化学分册, 2008, 44(1): 71～73
被引情况:
【1】沈劼,程欲晓,张琳,周宇艳,金樱华,郭争云,陈宁宁,鲜跃仲, "机械剥离石墨烯修饰电极检测食品接触材料中双酚A的迁移量",理化检验－化学分册 51, 765-769(2015)
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参考文献
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【5】Maudens K E,Zhang G F,Lambert W E. Quantitative analysis of twelve sulfonamides in honey after acidic hydrolysis by high-performance liquid chromatography with post-column derivatization and fluorescence detection[J]. J Chromatogr A,2004,1047(1):85-92．
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【10】Fu C G,Song L N,Fang Y Z. Determination of adenosine phosphate by co-electroosmotic capillary electrophoresis with amperometric detection[J]. Anal Chim Acta,1999,399:259-263．
【2】Mellado Romero A,Gómez Benito C,Martínez Calatayud J. Continuous-flow spectrophotometric determination of sulfadiazine by diazotisation with in situ preparation of nitrite[J]. Anal Chim Acta,1995(308):451-456．
【3】Siska Croubels,Pascal Wassink,Patric De Backer. Simultaneous determination of sulfadiazine and trimethoprim in animal feed by liquid chromatography with UV and tandem mass spectrometric detection[J]. Anal Chim Acta,2002(473):183-194．
【4】Msagati T A M,Nindi M M. Multiresidue determination of sulfonamides in a variety of biological matrices by supported liquid membrane with high pressure liquid chromatography-electrospray mass spectrometry detection[J]. Talanta,2004,64(1):87-100．
【5】Maudens K E,Zhang G F,Lambert W E. Quantitative analysis of twelve sulfonamides in honey after acidic hydrolysis by high-performance liquid chromatography with post-column derivatization and fluorescence detection[J]. J Chromatogr A,2004,1047(1):85-92．
【6】Loeffler Dirk. Ternes,Thomas A Determination of acidic pharmaceuticals,antibiotics and ivermectin in river sediment using liquid chromatography-tandem mass spectrometry[J]. J Chromatogr A,2003,1021(1/2):133-144．
【7】Catala Icardo M,Garcia Mateo J V,et al. Enhanced flow-injection-chemiluminometric determination of sulphonamides by on-line photochemical reaction[J]. Anal Chim Acta,2003,499(1/2):57-69．
【8】Nagaraja P,Yathirajan H S,Raju C R,et al. 3-Aminophenol as a novel coupling agent for the spectrophotometric determination of sulfonamide derivatives[J]. Farmaco,2003,58(12):1295-1300．
【9】方禹之,龚飞雁,刘晓峰,等.高效毛细管电泳-电化学检测对废水中优先监测酚类的测定研究[J].分析化学,1998,26(6):694-697．
【10】Fu C G,Song L N,Fang Y Z. Determination of adenosine phosphate by co-electroosmotic capillary electrophoresis with amperometric detection[J]. Anal Chim Acta,1999,399:259-263．
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