CdTe/CdS量子点共振瑞利散射光谱法测定细胞色素C
Resonance Rayleigh Scattering Spectrometric Determination of Cytochrome C by its Reaction with CdTe/CdS quantum dots
摘 要
以3-巯基丙酸为稳定剂,合成了具有特殊光学性质的水溶性核壳型CdTe/CdS量子点.基于细胞色素C对该量子点的共振瑞利散射有显著的增强作用,建立了一种快速检测细胞色素C的方法.在pH 7.0的磷酸盐缓冲液(PBS)中,量子点在385 nm波长处的共振瑞利散射的增强与细胞色素C浓度呈线性关系,线性范围为0.036~1.31 mg·L-1,检出限为10.8 μg·L-1.方法已用于人体血清中细胞色素C含量的测定,并用标准加入法测得方法的回收率在90.4%~93.2%之间.
共振瑞利散射
细胞色素C
CdTe/CdS core-shell quantum dots
Resonance rayleigh scattering
Cytochrome C
Abstract
Water soluble CdTe/CdS core-shell quantum dots,having special optical and spectral characteristics,were synthesized,using 3-mercaptopropionic acid as stabilizer.Based on the significant enhancement effect of cytochrome C on the resonance rayleigh scattering (RRS) of CdTe/CdS quantum dots,a new method for the determination of cytochrome C was proposed.Linear relationship between the enhanced RRS signals measured at 385 nm in a phosphate buffer of pH 7.0 and the concentration of cytochrome C was found in the range from 0.036 to 1.31 mg·L-1,with a detection limit of 10.8 μg·L-1.The proposed method has been applied to the analysis of 3 samples of human serum,and values of recovery determined by standard addition method were in the range from 90.4% to 93.2%.
中图分类号 O657.39
所属栏目 试验与研究
基金项目 国家自然科学基金(No.20475024)与聊城大学科研基金(X061003)项目资助
收稿日期 2007/8/6
修改稿日期
网络出版日期
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备注闫炜(1982-),女,山东济宁人,硕士研究生,主要研究方向为光谱电化学.
引用该论文: YAN Wei,ZHANG Ai-mei,WANG Huai-sheng. Resonance Rayleigh Scattering Spectrometric Determination of Cytochrome C by its Reaction with CdTe/CdS quantum dots[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(2): 107～110
闫炜,张爱梅,王怀生. CdTe/CdS量子点共振瑞利散射光谱法测定细胞色素C[J]. 理化检验－化学分册, 2008, 44(2): 107～110
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【3】Sandra M C,Russell H S,Catarina R O.Induction of cytochrome c-mediated apoptosis by amyloid β 25-35 requires functional mitochondria[J].Brain Research,2002,931(2):117-125．
【4】李任强,陈 瑶,江凤仪,等.微量动力学分光光度法检测细胞色素C制品的品质[J].分析科学学报,2005,21(4):463-464．
【5】朱民,施国跃,刘敏,等.ED/Au /I-复合物修饰电极在细胞色素C测定中的应用[J].华东师范大学学报,2003,1:68-73．
【6】杨传孝,李原芳,黄承志.丽春红G用于人血清样品中总蛋白的共振光散射测定[J].分析化学,2003,31(2):148-152．
【7】Liu S P,Liu Q.Resonance rayleigh-scattering method for the determination of proteins with monoazo dyes of chromotropic acid[J].Analytical Sciences,2001,17(2):239-242．
【8】Xu L.Surface passivation and enhanced quantum-size effect and photostability of coated CdSe/CdS nanocrystals[J].Physica E,2000,6(8):129-133．
【9】Cao Y W,Banin U.Growth and properties of semiconductor core/shell nanocrystals with InAs cores[J].J Am Chem Soc,2000,122:9692-9702．
【10】Bao H B,Gong Y J,Li Z,et al.Enhancement effect of illumination on the photoluminescence of water-soluble CdTe nanocrystals: toward highly fluorescent CdTe/CdS core-shell structure[J].Chem Mater,2004,16:3853-3859．
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