反相高效液相色谱法测定阿莫西林
RP-HPLC Determination of Amoxicillin
摘 要
采用反相高效液相色谱法测定阿莫西林并对测定条件及有关参数进行了试验.用Shim-Pack VP-ODS柱作色谱柱,以磷酸盐缓冲液(pH 5.0)及乙醇以90比10的混合液为流动相,流速为1.0 mL·min-1,在228 nm波长处测定阿莫西林的含量,其线性范围为0.045~0.60 g·L-1,相关系数为0.997 4.将方法应用于阿莫西林胶囊的测定,测定结果的相对标准偏差值小于0.6%,用标准加入法作回收试验,回收率结果在94.7%~101.3%之间.
阿莫西林
药剂
RP-HPLC
Amoxicillin
Drugs
Abstract
RP-HPLC was applied to the determination of amoxicillin in its drugs. Conditions and parameters of the method and instrument were studied and optimized. The Shim-Pack VP-ODS column (150 mm×4.6 mm,i.d. 5 μm) was used as the chromatographic column,and a mixed solution composed of the phosphate buffer (pH 5.0) and ethanol in the ratio of 90 to 10 (by volume) was used as the mobile phase. Flow-rate of the mobile phase was 1.0 mL·min-1. UV-detection at 228 nm was adopted. Linear relationship between the peak area (mV·s) and concentration of amoxidillin (g·L-1) was obtained in the range of 0.045-0.60 g·L-1 (r=0.997 4). In its application to the analysis of amoxicillin capsules,RSD′s calculated were <0.6%. Results of recovery test performed by the standard addition method were in the range of 94.7%-101.3%.
中图分类号 O657.7
所属栏目 工作简报
基金项目
收稿日期 2006/10/31
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备注李彦威(1959-),男,河北武安人,博士,教授,主要从事有机电化学、药物合成及分析的研究工作.
引用该论文: LI Yan-wei,GOU Hong-lei,YANG Xiu-hua,WANG Zhi-zhong. RP-HPLC Determination of Amoxicillin[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(3): 216～217
李彦威,勾洪磊,杨秀花,王志忠. 反相高效液相色谱法测定阿莫西林[J]. 理化检验－化学分册, 2008, 44(3): 216～217
被引情况:
【1】庞向东,刘艳,邓钦心,张华,江虹, "甲基紫褪色光度法测定阿莫西林的含量",理化检验－化学分册 47, 982-983(2011)
【2】江虹,舒亚林,张秀菊, "亚甲基蓝共振瑞利散射法测定阿莫西林的含量",理化检验－化学分册 47, 1063-1065(2011)
【3】江虹,冯默,张华, "荧光光度法测定阿莫西林",理化检验－化学分册 46, 1447-1449(2010)
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参考文献
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【11】Hoizey G,Lamiable D,Frances C,et al. Simultaneous determination of amoxicillin and clavulanic acid in human plasma by HPLC with UV detection[J]. J Pharm Biomed Anal,2002,30:661．
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【2】庞西英.阿莫西林胶囊含量2种测定方法的比较[J].药物分析杂志,1999,19(3):205．
【3】李媛,郎惠云,李惠娥,等.流动注射在线过滤稀释-原子吸收分光光度法测定阿莫西林[J].分析化学,2000,28(10):1252．
【4】任乃林,陈宜菲,韩大雄.极谱法测定阿莫西林胶囊的含量[J].分析测试技术与仪器,2004,10(1):50．
【5】范顺利,张 磊,买文宁,等.流动注射-化学发光法测定阿莫西林[J].理化检验-化学分册,2003,39(5):257．
【6】赵丽瑞.阿莫西林-铜络合物光度法测定阿莫西林[J].光谱实验室,1999,16(2):189．
【7】夏运岳,袁正希,陈幼亭.紫外光谱标准加入法测定阿莫西林片剂的含量[J].中国抗生素杂志,1997,22(5):392．
【8】程存归,应桃开.漫反射傅里叶变换红外光谱定量分析法测定阿莫西林胶囊的含量[J].中国抗生素杂志,1997,27(6):337．
【9】中华人民共和国药典委员会编.中华人民共和国药典(二部)[M].北京:化学工业出版社,2000:338-339．
【10】王雷,梁颖.HPLC法测定阿莫西林胶囊中阿莫西林的含量[J].天津药学,2000,12(3):69．
【11】Hoizey G,Lamiable D,Frances C,et al. Simultaneous determination of amoxicillin and clavulanic acid in human plasma by HPLC with UV detection[J]. J Pharm Biomed Anal,2002,30:661．
【12】Muth P,Metz R,Beck H,et al. Improved high-performance liquid chromatographic determination of amoxicillin in human plasma by means of column switching[J]. J Chromatogr A,1996,729:259．
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