分光光度法测定纤维素水解液中5-羟甲基糠醛和糠醛
Spectrophotometric Determination of 5-Hydroxyfurfural and Furfural in the Hydrolyzed Liquor of Cellulose
摘 要
5-羟甲基糠醛(5-HMF)和糠醛(FUR)是纤维素酸水解过程中产生的中间产物.此文提出了用硫代巴比酸作为衍生试剂并利用一阶导数分光光度法测定5-HMF和FUR的方法.利用零点交叉测量法在412 nm和428 nm波长下,可以同时确定5-HMF和FUR的含量,两者的质量浓度在0.4~3.2 mg·L-1范围内与一阶导数值呈线性关系.试验了方法的精密度和回收率,同时测定两组分的相对标准偏差(n=7)分别为2.23%和2.06%,回收率分别为96.0%和105.0%.
5-羟甲基糠醛
糠醛
硫代巴比酸
纤维素
水解
1st derivative spectrophotometry
5-Hydroxyfurfural
Furfural
Thiobarbituriuc acid
Cellulose
Hydrolysis
Abstract
First derivative spectrophotometry was used in the determination of 5-hydroxyfurfual and furfural,which were produced in the hydrolysis of cellulose as intermediate products. After reacted with thiobarbituricacid solution,and by the method of zero-crossing measurement,5-hydroxyfurfural was determined at the wavelength 412 nm and furfural determined at 428 nm. Linear relationships between the values of 1st derivative,dA/dλ412 and dA/dλ428,and the concentrations of both the compounds were obtained in the range of 0.4-3.2 mg·L-1. Precision and recovery of the method were tested,giving values of RSD′s (n=7) 2.23% and 2.06%,values of recovery 96.0% and 105.0% for 5-hydroxyfurfural and furfural respectively.
中图分类号 O657.31
所属栏目 工作简报
基金项目 河南省教育厅自然科学研究计划项目资助(200510459056)
收稿日期 2006/10/27
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备注常春(1973-),男,河南郑州人,博士研究生,研究方向为生物质资源利用.
引用该论文: CHANG Chun,MA Xiao-jian,CEN Pei-lin. Spectrophotometric Determination of 5-Hydroxyfurfural and Furfural in the Hydrolyzed Liquor of Cellulose[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(3): 223～225
常春,马晓建,岑沛霖. 分光光度法测定纤维素水解液中5-羟甲基糠醛和糠醛[J]. 理化检验－化学分册, 2008, 44(3): 223～225
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参考文献
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