流动注射-鲁米诺-铁氰化钾反应体系化学发光法测定依诺沙星
Flow Injection Chemiluminescence Determination of Enoxacin by the Reaction System of Luminol and Potassium Ferricyanide
摘 要
试验发现依诺沙星对鲁米诺与铁氰化钾之间的化学发光反应具有显著的增敏作用,而且当依诺沙星浓度在2.0×10-8~2.0×10-5mol·L-1范围内,与相应的化学发光增强信号值(ΔI)之间呈线性关系,其检出限(3S)为5.7×10-9mol·L-1,取1.0×10-6mol·L-1依诺沙星按试验方法测定11次,算得其相对标准偏差为1.75%.结合应用流动注射技术,提出了流动注射-化学发光法(FI-CL)测定药片中依诺沙星的方法,应用此方法分析了3个依诺沙星片剂试样,所测得的结果与其标示值相符.按标准加入法作了回收率试验,所得结果在96.7%~103.0%之间.
鲁米诺
铁氰化钾
依诺沙星
药物
Flow injection-chemiluminescence determination
Luminol
Potassium ferricyanide
Enoxacin
Drug
Abstract
It was found that the chemiluminescence (CL) reaction between luminol and potassium ferricyanide was significantly sensitized by enoxacin,and that linear relationship between the enhanced CL signal (ΔI) and the concentration of enoxacin was held in the range of 2.0×10-8-2.0×10-5mol·L-1 with a detection limit (3S) of 5.7×10-9mol·L-1. Precision of the method was tested at the concentration level of 1.0×10-6mol·L-1 for 11 determination,giving value of RSD 1.75%. By applying the flow injection (FI) technique to the CL reaction system,a new FI-CL method was proposed for the determination of enoxacin in enoxacin tablets. In analyzing 3 samples of the drug tablets,results obtained by this method were in conformity with the labelled values. Test for recovery was made by standard addition method and results found were in the range of 96.7%-103.0%.
中图分类号 O657.3
所属栏目 工作简报
基金项目 安徽省高校教师青年基金(2002JQ128),安徽省教育厅自然科学基金(2003kj146),国家自然科学基金(Nos.20271002,20490210)资助项目
收稿日期 2006/10/25
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备注李世凤(1975-),女,安徽蚌埠人,副教授,博士,主要从事化学发光及光谱分析.
引用该论文: LI Shi-feng,WEI Xian-wen,ZHU Chang-qing,XIAO Yan-ling,CHEN Hong-qi,ZHOU Yuan-hui. Flow Injection Chemiluminescence Determination of Enoxacin by the Reaction System of Luminol and Potassium Ferricyanide[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(3): 247～248
李世凤,魏先文,朱昌青,肖艳玲,陈红旗,周远辉. 流动注射-鲁米诺-铁氰化钾反应体系化学发光法测定依诺沙星[J]. 理化检验－化学分册, 2008, 44(3): 247～248
被引情况:
【1】周娟,金桂云,钟立群, "流动注射化学发光法测定葛根黄酮",理化检验－化学分册 51, 506-508(2015)
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参考文献
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【3】刘一品,杨小钢,李常胜.紫外分光光度法测定依诺沙星含量的改进[J].武汉大学学报,2001,22(4):367．
【4】杜黎明,曹玺珉,吴红艳.胶束增敏-同步荧光光谱法直接测定血浆中的依诺沙星[J].分析化学,2003,31(11):1330．
【5】钟爱国.铋酸盐-依诺沙星修饰碳糊电极的制作及应用[J].分析仪器,2002,1:16．
【6】卢文斌.高效液相色谱法测定依诺沙星片的含量[J].海峡药学,2003,15(3):24．
【7】曾玲,刘宁,傅建.HPLC法测定依诺沙星软膏的含量[J].中国抗生素杂志,1997,22(4):153．
【8】Vreea A T B,Baars M,Wijnands W J A. High-performance liquid chromatographic determination of fluoroquinolones[J]. J Chromatogr Sci,1985,343:449．
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