Fluorospectrophotometric Characteristic of the Intercombination of Rifampin with Bovine Serum Albumin
摘 要
应用荧光光度法研究了水溶液中利福平与牛血清白蛋白分子间的结合反应,讨论了利福平对蛋白质内源荧光的猝灭机理,测定了结合常数(KA=3.1×10-5L·mol-1)和结合位点数(n=1.06).依据Frster非辐射能量转移理论确定了供体-受体间的结合距离(r=3.89 nm)和能量转移效率,并用同步荧光技术考察了利福平对牛血清白蛋白构象的影响.
Abstract
Intermolecular combination of rifampin with bovine serum albumin (BSA) in aqueous solution was studied by fluorospectrophotometry. Mechanism of quenching of fluorescence of BSA by rifampin was discussed. Equilibrium constant of combination (KA) and number of combination site (n) were determined and values obtained were 3.1×10-5L·mol-1 and 1.06 respectively. By the Frster non-radiating energy transfer theory,the combination distance between the donor and acceptor as well as the efficiency of energy transfer were determined,and the value of combination distance (r) was found to be 3.89 nm. Effect of rifampin on the configuration of BSA was studied by the synchronous fluorospectrophotometry.
中图分类号 O657.39
所属栏目 工作简报
基金项目 聊城大学科研基金(X061003)
收稿日期 2006/10/12
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备注张爱梅(1948-)女,山东莘县人,教授,研究方向为分子光谱与生命科学.
引用该论文: ZHANG Ai-mei,QIN Yan-he,LI Yuan. Fluorospectrophotometric Characteristic of the Intercombination of Rifampin with Bovine Serum Albumin[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(3): 261~263
张爱梅,秦延河,李媛. 利福平和牛血清白蛋白相互结合作用的荧光光度特性[J]. 理化检验-化学分册, 2008, 44(3): 261~263
被引情况:
【1】周秋华,张红梅,文美,王彦卿, "荧光光谱法研究2,4,6-三氯苯酚与胰蛋白酶的相互作用",理化检验-化学分册 46, 1109-1113(2010)
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参考文献
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【3】张海容,郭祀远,李琳,等.荧光法研究司帕沙星与白蛋白的作用[J].光谱学与光谱分析,2001,21(6):829-832.
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【6】朱铿,童沈阳.吡哌酸、氟哌酸与HSA,IgG相互作用的研究[J].化学学报,1997,55:405-410.
【7】马春琪,李克安,赵凤林,等.牛血清白蛋白与铬天青S作用机理的研究[J].化学学报,1999,57:389-395.
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