喷他脒的电化学行为及其应用
Electrochemical Behavior of Pentamidine and Its Application
摘 要
采用循环伏安法研究了喷他脒在玻碳电极上的电化学行为,应用微分脉冲伏安法提出了喷他脒不需分离的直接测定方法.在0.1 mol·L-1(pH 8.5)磷酸二氢钠-磷酸氢二钠溶液中,喷他脒于电位-1.56 V(vs.SCE) 有一灵敏的还原波;试验证明该波是喷他脒的催化氢波,还原波峰电流与喷他脒浓度在2.0×10-5~8.0×10-4mol·L-1范围内呈线性关系,相关系数为0.997 3,检出限为1.6×10-5mol·L-1;采用标准加入法测定尿液中样品,回收率在95.1%~103.8%之间.
喷他脒
电化学行为
催化氢波
Derivative pulse voltammetry
Pentamidine
Electrochemical behavior
Catalytic hydrogen wave
Abstract
Electrochemical behavior of pentamidine (PTM) was studied by cyclic voltammetry.As shown by experimental results,in a phosphate buffer of pH 8.5,a sensitive reduction wave produced by PTM was observed at the potential -1.56 V (vs.SCE),which is proved to be a catalytic hydrogen wave.Linear relationship was held between peak current of the reduction wave and concentration of PTM in the range of 2.0×10-5-8.0×10-4mol·L-1 (r=0.997 3).Detection limit of the method was found to be 1.6×10-5mol·L-1.Based on these facts,a derivative pulse voltammetric method of direct determination of PTM without necessity of separation was proposed.Recovery test was performed by addition of standard PTM solution to a urine sample,and the results obtained were in the range of 95.1%-103.8%.
中图分类号 O657.1
所属栏目 试验与研究
基金项目 国家自然科学基金资助项目(20472076);河南省杰出青年科学基金资助项目(0512001400)
收稿日期 2007/10/10
修改稿日期
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备注屈凌波(1963-),男,河南省南阳市人,教授,博士,主要从事药物分析及药物分子设计研究.
引用该论文: YANG Ran,YANG Su-ling,QU Ling-bo,LI Jian-jun. Electrochemical Behavior of Pentamidine and Its Application[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(4): 304～306
杨冉,杨素玲,屈凌波,李建军. 喷他脒的电化学行为及其应用[J]. 理化检验－化学分册, 2008, 44(4): 304～306
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参考文献
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【2】Elson J D.The antibacterial and fungistatic properties of propamidine[J].J Infect Dis,1945,76:193-197．
【3】Vonderfecht S L,Miskuff S B,Wee S,et al.Protease inhibitors suppress the in vitro and in vivo replication of rotavirus[J].J Clin Invest,1988,82:2011-2016．
【4】Yeh T K,Dalton J T,Au J L S.High-performance liquid chromatographic determination of pentamidine in plasma[J].J Chromatogr (Biomedical Applications ),1993,622:255-260．
【5】Garzh M J,Rabanal B,Ortizb A I,et al.Determination of pentamidine in serum and urine by micellar electrokinetic chromatography[J].Journal of Chromatography B,1997,688:135-142．
【6】田新娟,宋俊峰.丹参酮A在碳糊电极上的伏安行为及其测定[J].分析化学,2006,34(9):1283-1286．
【7】Laviron E.General expression of the linear potential sweep voltammery in the case of diffusionless electrochemical systems[J].J Eletroanal Chem,1979,101:19-28．
【8】姚修仁,张立群.极谱催化波图谱[M].北京:地质出版社,1988:25．
【9】马淮凌,徐茂田,宋俊峰.催化氢波-单扫描示波极谱法测定克拉霉素[J].理化检验-化学分册,2005,41(3):156-158．
【10】曾泳准,李娟.辛可尼丁的催化氢波[J].分析试验室,1999,18(4):22-25．
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