SPE-RHPLC Determination of Polyhexamethylenebiguanide in Lens Cleaning-Disinfectant Solution
摘 要
建立了固相萃取-反相高效液相色谱法测定隐形眼镜护理液中聚胺丙基双胍的方法.用Spe-ed WCX固相萃取柱(500 mg/3 mL)将样品浓缩富集后,以Shiseido C18色谱柱(150 mm×4.6 mm,5 μm)为分离柱,以pH 4.0的乙腈-20 mmol·L-1乙酸铵(体积比16比84)为流动相,检测波长为235 nm,7 min内测定样品中聚胺丙基双胍.方法检出限(S/N=3)为0.046 mg·L-1,相对标准偏差为3.3%(n=7),线性范围为100~800 mg·L-1,加标回收率93.5%~110.9%.
Abstract
SPE-RHPLC was applied to the determination of polyhexamethylenebiguanide (PHMBG) in lens cleaning-disinfectant solution.PHMBG was enriched on the Spe-ed WCX solid phase extraction column and separated on the shiseido C18 column.A mixed solution of acetonitrile and 20 mmol·L-1 ammonium acetate (mixed at a ratio of 16 to 84) at pH 4.0 was used as mobile phase.UV-detection at 235 nm was adopted in the determination.Linear relationship between values of peak area and concentration of PHMBG was obtained in the range of 100-800 mg·L-1,and detection limit (S/N=3) of the method was found to be 0.046 mg·L-1.Tests for recovery and precision were performed and results of recovery in the range from 93.5% to 110.9% and value of RSD′s (n=7) of 3.3% were obtained.Whole process of a determination was completed within 7 min.
中图分类号 O657.7
所属栏目 工作简报
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收稿日期 2006/12/30
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备注丁晓静(1968-),女,河北望都县人,副主任技师,博士,主要研究方向为卫生检验.
引用该论文: DING Xiao-jing,CHE Yi-ping,WANG Ying,LIU Peng-yan. SPE-RHPLC Determination of Polyhexamethylenebiguanide in Lens Cleaning-Disinfectant Solution[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(4): 359~360
丁晓静,车宜平,王颖,刘芃岩. 固相萃取-反相高效液相色谱法测定隐形眼镜护理液中聚胺丙基双胍[J]. 理化检验-化学分册, 2008, 44(4): 359~360
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参考文献
【1】卫生部卫生法制与监督司.消毒技术规范2002版[M].北京:中华人民共和国卫生部,2002:123-124.
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【2】Che Yiping,Ding Xiaojing,Zhao Shan,et al.Rapid determination of polyhexamethylenebiguanide in compound chemical disinfectants by reversed-phase high performance liquid chromatography[J].国际网上化学学报,2005,7(8):078055ne.
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