Application of Response Surface Experimental Designing to the Study on the Chromatographic Behavior of Carbofuran in Its GC Determination
摘 要
应用响应曲面试验设计法研究了气相色谱不同的进样方式,包括常规分流不分流进样以及柱头进样对克百威色谱行为的影响.通过构建模型,对试验的影响因子包括进样口温度、初始柱温和载气流速对克百威色谱行为的相对重要性进行了评价.并利用面积归一化方法,对进样口温度和初始柱温对克百威分解的影响进行了定量评价和探讨.结果表明:在常规进样方式中,进样口温度和初始柱温是影响克百威分解的主要因素.通过色谱条件的优化,解决了常规进样不能解决的克百威的热分解问题.
Abstract
Effect of different modes of sample introduction,including the conventional separate-streaming or non-separate-streaming sample introduction and the head-column sample introduction,on the chromatographic behavior of carbofuran in the GC determination was studied by the method of response surface experimental designing.By studying with the mathematical model established,relative significance of the influential factors,i.e.temperature at the sample inlet,initial column temperature and flow-rate of carrier gas,as related to the thermodecomposition of carbofuran was evaluated.As judged by area normalization,the effects of sample inlet temperature and initial column temperature were evaluated quantitatively,and it was concluded that these 2 factors were the main cause for the thermodecomposition of carbofuran,which was proved to be unavoidable in the conventional mode of sample introduction,even under the optimized working conditions.
中图分类号 O657.7
所属栏目 试验与研究
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收稿日期 2007/12/5
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备注丁振华(1981-),男,安徽人,工程师,主要从事食品安全与产品质量监督检验和研究工作.
引用该论文: DING Zhen-hua,HU Yan-yun,SHENG Xuan,ZHENG Ping. Application of Response Surface Experimental Designing to the Study on the Chromatographic Behavior of Carbofuran in Its GC Determination[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(5): 395~398
丁振华,胡艳云,盛旋,郑平. 应用响应曲面试验设计法研究克百威气相色谱测定中的色谱行为[J]. 理化检验-化学分册, 2008, 44(5): 395~398
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参考文献
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【3】Rossi S,Dalpero A P,Ghini S,et al.Multiresidual method for the gas chromatographic analysis of pesticides in honeybees cleaned by gel permeation chromatography[J].J Chromatogr A,2001,905:223.
【4】Climent M J,Miranda M A.Gas chromatographic-mass spectrometric study of photodegradation of carbamate pesticides[J].J Chromatogr A,1996,738:225.
【5】Pinzauti S,Gratteri P,Furlanetto S,et al.Experimental design in the development of voltammetric method for the assay of omeprazole[J].J Pharm Biomed,1996,14:881.
【6】Carlson R.Design and Optimization in Organic synthesis[M].Elsevier,Amsterdam,1992.
【7】Ginkel S V,Sung S,Lay J J.Biohydrogen Production as a Function of pH and Substrate Concentration[J].Environ Sci Technol,2001,35:4726.关于开展材料成分、化验(检验)人员职业资格考核培训通知材料成分,化验(检验)人员职业资格考核培训即将在南京市开班.授课以应用为主线,理论为基础,内容实用,取材广泛,涵盖材料成分,化验(检验)应知应会的全部基础专业知识.
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