单糖乙酰化条件的优化
Optimization of Conditions for Acetylation of Monosaccharides
摘 要
利用气相色谱-质谱联用技术(GC-MS)检测5种常见单糖(木糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖)的乙酰化产物含量.为在预衍生化中取得较高的效率,采用正交试验综合考察了还原时间、还原温度、乙酰化温度、乙酰化时间等因素对单糖回收率的影响及规律.结果表明,影响最为显著的因子为还原温度和乙酰化时间.总结出了单糖衍生化最佳的反应条件为还原温度50 ℃、还原时间2.5 h;乙酰化温度90 ℃、乙酰化时间2.5 h.
乙酰化
单糖
正交试验
Hyphenation of GC and MS
Monosaccharides
Acetylation
Orthogonal experimental design
Abstract
Hyphenated GC and MS was used in the determination of acetylation products of 5 common monosaccharides,i.e.,xylose,arabinose,mannose,glucose and galactose.For obtaining high efficiency of pre-derivatization,conditions for acetylation,including the time and temperature of reduction,time and temperature of acetylation and etc.,were studied and optimized by using the orthogonal experimental designing method.It was found that the temperature of reduction and time of acetylation were found to be the significant factors influential to the recovery of monosaccharides.The optimized acetylation conditions were found as follows:① temperature of reduction:50 ℃;② time of reduction:2.5 h;③ temperature of acetylation:90 ℃ and ④ time of acetylation 2.5 h.
中图分类号 O657.63
所属栏目 工作简报
基金项目 安徽省自然科学基金项目(No.03043301);安徽大学211工程学术创新团队、安徽省中药研究与开发重点实验室资助
收稿日期 2007/1/23
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备注廖洪梅(1975-),女,安徽阜阳人,硕士研究生,主要进行多糖方向的研究.
引用该论文: LIAO Hong-mei,DAI Ling,WEI Xiao-fei,WANG Zhi-yuan,ZHANG Kai. Optimization of Conditions for Acetylation of Monosaccharides[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(5): 441～443
廖洪梅,戴玲,魏晓飞,王志远,张凯. 单糖乙酰化条件的优化[J]. 理化检验－化学分册, 2008, 44(5): 441～443
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参考文献
【1】左绍远,万顺康,狄勇,等.钝顶螺旋藻多糖降血糖调血脂实验研究[J].中国生化药物杂志,2000,21(6):289．
【2】Brecker Lothar,Wicklein Daniel,Moll Hermann,et al.Structural and immunological properties of arabinogalactan polysaccharides from pollen of timothy grass (Phleum pratense L.)[J].Carbohydrate Research,2005,340(4):657．
【3】Mei Xiangdong,Heng Linsen,Fu Mingkun,et al.Synthesis of β-D-Glcp-(1→3)-[β- D-Glcp-(1→6)]-β-D-Glcp-(1→3)-β-D-Glcp-(1→6)-[β-D-Galp-(1→4)-β-D-Glcp-(1→3)]-β-D-GlcpOLauryl,an oligosaccharide with anti-tumor activity[J].Carbohydrate Research,2005,340(15):2345．
【4】胡磊,郭蓓,王乐,等.分析植物组织中海藻糖的气质联用及毛细管气相色谱法[J].植物生理学通讯,2004,40(4):474．
【5】Sudhamani S R,Tharanathan R N,Prasad M S.Isolation and characterization of an extracellular polysaccharide from Pseudomonas caryophylli[J].Carbohydrate Polymers,2004,56:423．
【6】张惟杰.复合多糖生化研究技术[M].杭州:浙江大学出版社,1997:140．
【7】Michael E Quigley,Geoffrey J Hudson,Hans N Englyst.Determination of resistant short-chain carbohydrates (non-digestible oligosaccharides) using gas-liquid chromatography[J].Food Chemistry,1999,65:381．
【8】陈文峰,蒋凌月,徐丽华,等.气质联用定量分析放线菌全细胞单糖方法的研究[J].微生物学通报,2000,27(6):416．
【9】陈汝贤,徐桂云,张瑞,等.用毛细管气相色谱法测定多糖中单糖的组成[J].分析测试学报,2002,21(1):68．
【10】Qin Chuangguang,Huang Kaixun,Xu Huibi.Isolation and characterization of a novel polysaccharide from the mucus of the loach,Misgurnus anguillicaudatus[J].Carbohydrate Polymers,2002,49:367．
【11】郑雪红,郑爱榕,陈祖峰.气相色谱法分析海洋胶体多糖中的单糖组成[J].分析测试学报,2004,23(4):58．
【2】Brecker Lothar,Wicklein Daniel,Moll Hermann,et al.Structural and immunological properties of arabinogalactan polysaccharides from pollen of timothy grass (Phleum pratense L.)[J].Carbohydrate Research,2005,340(4):657．
【3】Mei Xiangdong,Heng Linsen,Fu Mingkun,et al.Synthesis of β-D-Glcp-(1→3)-[β- D-Glcp-(1→6)]-β-D-Glcp-(1→3)-β-D-Glcp-(1→6)-[β-D-Galp-(1→4)-β-D-Glcp-(1→3)]-β-D-GlcpOLauryl,an oligosaccharide with anti-tumor activity[J].Carbohydrate Research,2005,340(15):2345．
【4】胡磊,郭蓓,王乐,等.分析植物组织中海藻糖的气质联用及毛细管气相色谱法[J].植物生理学通讯,2004,40(4):474．
【5】Sudhamani S R,Tharanathan R N,Prasad M S.Isolation and characterization of an extracellular polysaccharide from Pseudomonas caryophylli[J].Carbohydrate Polymers,2004,56:423．
【6】张惟杰.复合多糖生化研究技术[M].杭州:浙江大学出版社,1997:140．
【7】Michael E Quigley,Geoffrey J Hudson,Hans N Englyst.Determination of resistant short-chain carbohydrates (non-digestible oligosaccharides) using gas-liquid chromatography[J].Food Chemistry,1999,65:381．
【8】陈文峰,蒋凌月,徐丽华,等.气质联用定量分析放线菌全细胞单糖方法的研究[J].微生物学通报,2000,27(6):416．
【9】陈汝贤,徐桂云,张瑞,等.用毛细管气相色谱法测定多糖中单糖的组成[J].分析测试学报,2002,21(1):68．
【10】Qin Chuangguang,Huang Kaixun,Xu Huibi.Isolation and characterization of a novel polysaccharide from the mucus of the loach,Misgurnus anguillicaudatus[J].Carbohydrate Polymers,2002,49:367．
【11】郑雪红,郑爱榕,陈祖峰.气相色谱法分析海洋胶体多糖中的单糖组成[J].分析测试学报,2004,23(4):58．
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