细胞色素c/L-半胱氨酸修饰金电极检测亚硝酸根
Voltammetric Determination of Nitrite Radical Using the Modified Electrode L-Cys/Au Immobilized with Cytochrome c
摘 要
采用循环伏安法、交流阻抗法研究了组装在L-半胱氨酸(L-Cys)修饰金电极上的细胞色素c(Cyt c)电化学行为;采用电化学方法以及紫外-可见光谱对电极进行表征.结果表明:通过静电吸附作用组装在L-Cys修饰金电极上的细胞色素c保持了良好的生物和电化学活性,用Cyt c/L-Cys修饰金电极检测亚硝酸根,响应电流与亚硝酸根浓度在5.0×10-6~4.5×10-4mol·L-1范围内呈线性关系,线性回归方程为Ip=0.031 c+8.165×10-6,相关系数为0.999 5,检出限(S/N=3)为1.5×10-7mol·L-1.电极用于模拟样品及咸菜样品中亚硝酸根的测定,回收率为89.0%~116.0%.
修饰金电极
L-半胱氨酸
细胞色素c
亚硝酸根
Voltammetry
Modified Au electrode
L-Cysteine
Cytochrome c
Nitrite
Abstract
Electrochemical behavior of cytochrome c immobilized on the modified electrode of L-cystein/Au(abbreviated as Cyt.c/L-Cys/Au) was studied by cyclic voltammetry and alternating impedance,and the modified electrode was characterized by electrochemical methods and UV-VIS spectrophotometry.As shown by experimental results,cytochrome c,immobilized electrostatically on the L-cysteine/Au electrode,behaved to keep its biological and electrochemical activity in a good state.The modified electrode Cyt.c/L-Cys/Au was used as working electrode in the voltammetric determination of nitrite radical,and linear relationship between the values of response peak current(Ip) and concentration of NO-2 ion was obtained in the range from 5.0×10-6 to 4.5×10-4mol·L-1 in respective to the linear regression equation of Ip=0.031 c+8.165×10-6(r=0.999 5).Detection limit(S/N=3) of the method was found to be 1.5×10-7mol·L-1.Simulated samples and samples of pickles were analyzed by the proposed method,giving values of recovery in the range of 89.0%-116.0%.
中图分类号 O646
所属栏目 试验与研究
基金项目 国家高技术研究发展计划(813)项目(06Z4),上海市教委项目(06ZZ17)
收稿日期 2008/2/1
修改稿日期
网络出版日期
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备注李梦瑶(1983-),女,上海人,硕士研究生,主要研究方向为电化学生物传感技术.
引用该论文: LI Meng-yao,KONG Ling-mi,ZHU Pei-si,PENG Bin,HUANG Shan-sheng,YUAN Yin-xiang. Voltammetric Determination of Nitrite Radical Using the Modified Electrode L-Cys/Au Immobilized with Cytochrome c[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(6): 495～498
李梦瑶,孔令蜜,朱佩思,彭斌,黄杉生,袁寅祥. 细胞色素c/L-半胱氨酸修饰金电极检测亚硝酸根[J]. 理化检验－化学分册, 2008, 44(6): 495～498
被引情况:
【1】吴慧庆,苏耀东,王敏, "流动注射-聚中性红膜修饰电极安培法测定痕量亚硝酸根",理化检验－化学分册 46, 232-234(2010)
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参考文献
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【17】孙一新,王升富.细胞色素c在L-半胱氨酸自组装膜电极上的电化学行为[J].应用化学,2005,22:295-299．
【2】Yeh P,Kuwana T.Reversible Electrode Reaction of Cytochrome c[J].Chem Lett,1977,10:1145-1148．
【3】Matsuda N,Santos J H,Takats A,et al.Spectroelectrochemical studies on surface immobilized cytochrome c on ITO electrode by slab optical waveguide spectroscopy[J].Thin Solid Films,2003,438-439:403-406．
【4】Marken F,Paddon C A,Asogen D.Direct cytochrome c electrochemistry at boron-doped diamond electrodes[J].Electrochem Commun,2002,4:62-66．
【5】Feng J J,Zhao G,Xu J J,et al.Direct electrochemistry and electrocatalysis of heme proteins immobilized on gold nanoparticles stabilized by chitosan[J].Anal Biochem,2005,342:280-286．
【6】Wu Y,Hu S S.Voltammetric investigation of cytochrome c on gold coated with a self-assembled glutathione monolayer[J].Bioelectrochemistry,2006,68:105-112．
【7】Park H,Park J S,Shim Y B.Electrochemical and in situ UV-Visible spectroscopic behavior of cytochrome c at a cardiolipin-modified electrode[J].J Electroanal Chem,2001,514:67-74．
【8】Mckenzie K J,Marken F,Opallo M.TiO2 phytate films as hosts and conduit for cytochrome c electrochemistry[J].Bioelectrochemistry,2005,66:41-47．
【9】Ding X Q,Hu J B,Li Q L.Direct electrochemistry and superficial characterization of DNA-cytochrome c-MUA films on chemically modified gold surface[J].Talanta,2006,68:653-658．
【10】Zhong T S,Qu Y X,Huang S S,et al.Electrochemical studies of cytochrome c on gold electrodes with promotor of humic acid and 4-aminothiophenol[J].Microchim Acta,2007,158:291-297．
【11】Wang S F,Du D,Zou Q C.Electrochemical behavior of epinephrine at L-cysteine self-assembled monolayers modified gold electrode[J].Talanta,2002,57:687-692．
【12】Souza F D,Rogers L M,ODell E S.Immobilization and electrochemical redox behavior of cytochrome c on fullerene film-modified electrodes[J].Bioelectrochemistry,2005,66:35-40．
【13】Liu Y C,Cui S Q,Zhao J,et al.Direct electrochemistry behavior of cytochrome c/L-cysteine modified electrode and its electrocatalytic oxidation to nitric oxide[J].Bioelectrochemistry,2007,70:416-420．
【14】刘云春,崔士强,杨周生.细胞色素C/L-半胱氨酸修饰电极的电化学行为研究[J].安徽师范大学学报:自然科学版,2006,3:255-257．
【15】Niu L M,Li N B,Kong W J.Electrochemical behavior of uric acid at a penicillamine self-assembled gold electrode[J].Microchimica Acta,2007,159:57-63．
【16】Irace G,Bismuto E,Savy F,et al.A cytosolic flavin-containing enzyme catalyzing reduction of cytochrome c in Trypanosoma cruzi: Kinetic studies with cytochrome c as substrate[J].Arch Biochem Biophys,1986,241:45-49．
【17】孙一新,王升富.细胞色素c在L-半胱氨酸自组装膜电极上的电化学行为[J].应用化学,2005,22:295-299．
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