应用高效液相色谱法测定了保健食品中他达拉非、西地那非、伐地那非等三种违禁药物含量.采用ODS色谱柱,乙腈-0.05 mol·L-1三乙胺(40+60)为流动相,流速1.0 mL·min-1,二极管阵列检测器,检测波长290 nm.根据保留时间、光谱图定性,峰高或峰面积定量.该方法三种药物组分在0.5~100.0 mg·L-1浓度范围内呈线性关系,他达拉非和西地那非的检出限为0.3 mg·L-1,伐地那非的检出限为0.4 mg·L-1,相对标准偏差为1.5%~2.0%,回收率为95.8%~107.0%.

HPLC was applied to the determination of 3 contraband drugs,i.e.,tadalafil,sildenafil and vardenafil,in health-care foodstuffs.Prodigy 5 μ ODS 3 column(250 mm×4.6 mm) was used as stationary phase,and a mixture of acetonitrile and 0.05 mol·L-1 triethylamine solution,mixed in the ratio of 40 to 60,was used as mobile phase,with a flow-rate of 1.0 mL·min-1.UV-detection at 290 nm with diode array detector(DAD) was adopted in the determination.Qualitative analysis was performed on the base of retention time and with reference to the standard spectra.Linear relationships between values of peak area and concentration of all the 3 drugs were found in the range of 0.5-100.0 mg·L-1.Detection limits(S/N=3) found for tadalafil and sildenafil were 0.3 mg·L-1,and for vardenafil was 0.4 mg·L-1.Precision and recovery were tested on known samples,and values of RSD′s(n=6) found were in the range of 1.5%-2.0% and values of recovery obtained were in the range from 95.8% to 107.0%.