HRP-纳米金/纳米硫化镉/聚天青Ⅰ修饰玻碳电极的制备及用作过氧化氢生物传感器
Preparation of HRP-nano Au/nano CdS/PolyazureⅠ Modified Glassy Carbon Electrode and It Use as Biosensor for Determination of Hydrogen Peroxide
摘 要
以玻碳电极为基底,电聚合一层表面均匀的带正电性的聚天青Ⅰ膜,再通过静电作用吸附一层带负电性的具有大比表面积的纳米硫化镉来固定纳米金和辣根过氧化物酶(HRP)的复合物,制备出性能良好的过氧化氢生物传感器.采用循环伏安法(CV)和计时电流法对该生物传感器的性能进行了研究.试验表明:该方法不仅增加了酶的吸附量,还有效保持了酶的生物催化活性,此生物传感器对过氧化氢浓度在4.0×10-7~1.2×10-3mol·L-1范围内呈线性关系,检出限为1.4×10-7mol·L-1.
天青Ⅰ
纳米金
纳米硫化镉
辣根过氧化物酶
Modified GCE
Azure Ⅰ
Gold nanoparticles
CdS nanoparticles
Horseradish peroxidase
Abstract
A homogeneous film of positively charged polyazure Ⅰ [abbreviated as (PAZ Ⅰ)+] was electrochemically polymerised and immobilized on the surface of glassy carbon electrode (GCE),and a layer of CdS nanoparticles with large specific surface and negative charge (nano CdS-) was then adsorbed on the surface of (PAZ Ⅰ)+ film through electrostatic action.Preparation of the enzyme modified electrode,used as biosensor for determination of H2O2,was finally completed by immobilization of the complex compound of horseradish peroxidase (HRP) and gold nanopariticles (nano Au) on to the surface of nano CdS/PAZ Ⅰ/GCE.The electrochemical property of the biosensor was studied by cyclic voltammetry and chronoamperometry.It was shown by the experimental results that through the process of preparation adopted,larger amount of HRP was immobilized and effective maintainance of the electrocatalytic activity was achieved by the application of nanotechnology,and that speedy response was specified as another feature of the biosensor.Linear relationship was obtained between the values of reductive peak current and concentration of H2O2 in the range of 4.0×10-7-1.2×10-3mol·L-1 with a detection limit of 1.4×10-7mol·L-1.
中图分类号 O657
所属栏目 试验与研究
基金项目 国家自然科学基金资助项目(20675064);重庆市自然科学基金资助项目(CSTC-2005 BB 4100)
收稿日期 2008/1/16
修改稿日期
网络出版日期
作者单位点击查看
备注李华刚(1959-),男,贵州遵义人,副教授,主要从事电化学及生物传感器的研究.
引用该论文: LI Hua-gang,YUAN Ruo,CHAI Ya-qin,MIAO Xiang-min,ZHUO Ying,HONG Cheng-lin. Preparation of HRP-nano Au/nano CdS/PolyazureⅠ Modified Glassy Carbon Electrode and It Use as Biosensor for Determination of Hydrogen Peroxide[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(8): 701～704
李华刚,袁若,柴雅琴,苗向敏,卓颖,洪成林. HRP-纳米金/纳米硫化镉/聚天青Ⅰ修饰玻碳电极的制备及用作过氧化氢生物传感器[J]. 理化检验－化学分册, 2008, 44(8): 701～704
被引情况:
【1】马美萍,连国军,曹建明, "辣根过氧化物酶/多壁碳纳米管修饰铂电极生物传感器用于过氧化氢的循环伏安测定",理化检验－化学分册 47, 278-280(2011)
【2】黄小梅,邓祥,吴狄,唐静, "用纳米铂-多壁碳纳米管-壳聚糖修饰玻碳电极计时电流法测定过氧化氢",理化检验－化学分册 49, 1042-1045(2013)
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【2】李峰,周延秀,朱果逸.流动注射-鲁米诺-高碘酸钾-过氧化氢体系的化学发光行为及其应用[J].分析化学,1997,25(11):1318-1320．
【3】胡俊明,石文鹏,林少彬.高效液相色谱法测定化妆品中过氧化氢的方法研究[J].中国卫生检验杂志,2003,13(5):593-596．
【4】崔莉凤,孟凡生.多糖激活的酪氨酸酶生物电极测定酚类的研究[J].理化检验-化学分册,2003,39(11):629-632．
【5】李亚卓,张素霞,李晓芳,等.基于溶胶-凝胶技术的聚烯丙胺基二茂铁化学修饰电极的组装及其对抗坏血酸的电催化氧化[J].高等学校化学学报,2003,24(8):1373-1376．
【6】穆绍林,杨一飞,谭志安.过氧化氢在磺酸二茂铁掺杂的聚苯胺上的电催化氧化[J].物理化学学报,2003,19(7):588-592．
【7】黎雪莲,袁若,柴雅琴,等.基于静电吸附甲苯胺蓝和纳米金固定过氧化物酶生物传感器的研究[J].分析化学,2006,34(3):389-392．
【8】王明艳,许兴友,高键,等.新型双核铜配合物修饰玻碳电极对抗坏血酸的电催化作用及其测定[J].理化检验-化学分册,2006,42(12):984-987．
【9】董绍俊,车广礼,谢远武,等.化学修饰电极[M].北京:科学出版社,1995．
【10】Crumbliss A L,Perine S C,Stonehuerner J,et al.Colloidal gold as a biocompatible immobilization matrix suitable for the Fabrica-tion of enzyme electrodes by electrodeposition[J].Biotech Bioeng,1992,4:483-490．
【11】张娟,徐静娟,陈洪渊.基于SiO2纳米粒子固定辣根过氧化物酶的生物传感器[J].高等学校化学学报,2004,25(4):614-617．
【12】Zhu X L,Yuri I,Gan X,et al.Electrochemical study of the effect of nano-Zinc oxide on microperoxidase and its application to more sensitive hydrogen peroxide biosensor preparation[J].Biosens Bioelectron,2007,22:1600-1604．
【13】Chen S H,Yuan R,Chai Y Q,et al.Amperometric third-generation hydrogen peroxide biosensor based on the immobilization of hemoglobin on multiwall carbon nanotubes and gold colloidal nanoparticles[J].Biosens Bioelectron,2007,22:1268-1274．
【14】Liu Y,Yuan R,Chai Y Q,et al.Direct electrochemistry of horseradish peroxidase immobilized on gold colloid／cysteine／nation-modified platinum disk electrode[J].Sens Actuators B,2006,115:109-115．
【15】张凌燕,袁若,柴雅琴,等.基于辣根过氧化物酶/纳米金/辣根过氧化物酶/多壁纳米碳管修饰的过氧化氢生物传感器的研究[J].化学学报,2006,64(16):1711-1715．
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【18】周锋,马强,唐亚平,等.硫化镉纳米粒子的制备[J].材料工程,2004(10):42-45．
【19】Slot J M,Geuze H J.A New method of prepare gold probes for multiple labeling cytochemistry[J].Cell Bio,1985,38:87-93．
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