通过超声诱导法提取土豆浸取液中的多酚氧化酶.收集上层清液与纯石墨粉混合,经阴干并与液态石蜡研磨均匀后,将此混合物装入空心碳电极中,由此制成多酚氧化酶电化学发光生物传感器.以此传感器对肾上腺素进行电化学发光检测,肾上腺素在多酚氧化酶存在下被溶液中的溶解氧所氧化而产生过氧化氢,而所产生的过氧化氢与鲁米诺反应产生化学发光,此乃生化传感器的作用机理.由于肾上腺素的存在而使化学发光强度的增加与其质量浓度在2×10-4~3×10-3g·L-1范围内呈线性关系;该方法的检出限为6.0×10-5g·L-1.对4.0×10-4g·L-1肾上腺素分别测定8次,所得结果的相对标准偏差小于5%.用此方法分析了肾上腺素注射液样品,测得结果与标准值相符.在此注射液的基础上作加标回收试验,测得回收率在94%~99%之间.

Polyphenol oxidase in potato was extracted by ultrasonic induction in water suspension.The surpernatant layer was collected and mixed with pure graphite powder.The mixture,after air-drying and homogeneous grinding with liquid paraffin,was filled into hollow carbon rod.An electro-chemiluminescence (ECL) biosensor was thus prepared.This ECL biosensor was used for chemiluminescence (CL) detection of epinephrine through the CL reaction between luminol and hydrogen peroxide which was produced by oxidation of epinephrine via dissolved oxygen in the presence of polyphenol oxidase.Linear relationship was obtained between the magnitude of enhanced CL intensity and concentration of epinephrine in the range of 2×10-4-3×10-3g·L-1,with a detection limit of 6×10-5g·L-1.Precision of the method was tested at the concentration level of 4.0×10-4g·L-1,values of RSD′s (n=8) obtained were less than 5%.The proposed method was applied to the assay of epinephrine injections,giving results in consistency with the labelled values.Test for recovery was made by adding standard solution of epinphrine to the injection samples,and values of recovery found were in the range of 94%-99%.