毛细管电泳非接触电导检测分离测定水杨酸及其羟基化产物
Separation and Determination of Salicylic Acid and Its Hydroxylating Products by Capillary Electrophoresis with Non-contact Conductivity Detection
摘 要
利用自组装的毛细管电泳非接触电导检测装置建立了水杨酸(SA,羟基自由基捕获剂)及其羟基化产物2,3-二羟基苯甲酸(2,3-DHBA)和2,5-二羟基苯甲酸(2,5-DHBA)的分离测定方法.在最佳条件下10 min内即可完成分离,其线性范围为0.03~15.0 mg·L-1L-1,检出限(S/N=3)为0.01 mg·L-1,对0.7 mg·L-1混合溶液进行测定,SA、2,3-DHBA和2,5-DHBA峰面积的相对标准偏差(n=8)依次为2.9%,3.2%,3.6%.
非接触电导检测
水杨酸
羟自由基
羟基化产物
Capillary electrophoresis
Non-contact conductivity detection
Salicylic acid
Hydroxyl free redical
Hydroxylation
Abstract
Salicylic acid (SA),a common siezing agent for hydroxyl free radical (·OH),and its hydroxylating products with the ·OH free radical,i.e.,2,3-dihydroxybenzoic acid (2,3-DHBA) and 2,5-dihydroxybenzoic acid (2,5-DHBA),were separated and determined by capillary electrophoresis with non-contact conductivity detection. Under optimum conditions,the above mentioned compounds were separated and detected within 10 min. Linear relationship between response signal and concentration of either of the 3 compounds was kept in the range of 0.03-15.0 mg·L-1,with detection limits of 0.01 mg·L-1. Test for precision was made at the concentration level of 0.7 mg·L-1 of each of the 3 compounds with 8 determinations,value of RSD′s for measurements of peak area were found to be 2.9% (for SA),3.2% (for 2,3-DHBA) and 3.6% (for 2,5-DHBA).
中图分类号 O657
所属栏目 工作简报
基金项目 国家自然科学基金(No.20675036),山东省自然科学基金(Y2006B04)和山东省“泰山学者”建设工程专项经费
收稿日期 2007/4/16
修改稿日期
网络出版日期
作者单位点击查看
备注赵芳(1981-),女,山东淄博人,硕士研究生,从事毛细管电泳研究.
引用该论文: ZHAO Fang,WANG Li-xin,FU Chong-gang. Separation and Determination of Salicylic Acid and Its Hydroxylating Products by Capillary Electrophoresis with Non-contact Conductivity Detection[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(9): 848～850
赵芳,王立新,傅崇岗. 毛细管电泳非接触电导检测分离测定水杨酸及其羟基化产物[J]. 理化检验－化学分册, 2008, 44(9): 848～850
共有人对该论文发表了看法，其中：
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】Grootveld M,Halliwell B. 2,3-Dihydroxybenzoic acid is a product of human aspirin metabolism[J]. Biochem Pharmacology,1988,37(2):271-280．
【2】Coudray C,Talla M,Martin S,et al. High-performance liquid chromatography-electrochemical determination of salicylate hydroxylation products as an in vivo marker of oxidative stress[J]. Anal Biochem,1995,227(1):101-111．
【3】Bergh V,Vanhees I,De-Boer R,et al. Identification of the oxidation products of the reaction between α-pinene and hydroxyl radicals by gas and high-performance liquid chromatography with mass spectrometric detection[J]. J Chromatogr A,2000,896(1/2):135-148．
【4】任信荣,邵可声,唐孝炎.高效液相色谱法测定水杨酸及其羟基化产物[J].色谱,2001,19(2):191-192．
【5】Jen F J,Meei F L,Yang T C. Determination of hydroxyl radicals in an advanced oxidation process with salicylic acid trapping and liquid chromatography[J]. J Chromatogr A,1998,796(2):283-288．
【6】Coolen S A J,Huf F A,Reijenga J C. Determination of free radical reaction products and metabolites of salicylic acid using capillary electrophoresis and micellar electrokinetic chromatography[J]. J Chromatogr B,1998,717(1/2):119-126．
【7】Wang Q J,Ding F,Zhu N N,et al. Determination of hydroxyl radical by capillary zone electrophoresis with amperometric detection[J]. J Chromatogr A,2003,1016(1):123-128．
【8】Ai S Y,Wang Q J,Li H,et al. Study on production of free hydroxyl radical and its reaction with salicylic acid at lead dioxide electrode[J]. J Electroanal Chem,2005,578(2):223-229．
【9】陈义.毛细管电泳技术及应用[M].北京:化学工业出版社,2000:47．
【10】Halliwell B,Grootved M. Aromatic hydroxylation as a potential measure of hydroxyl-radical formation in vivo. Identification of hydroxylated derivatives of salicylate in human body fluids[J]. J Biochem,1986,237(2):499-504．
【2】Coudray C,Talla M,Martin S,et al. High-performance liquid chromatography-electrochemical determination of salicylate hydroxylation products as an in vivo marker of oxidative stress[J]. Anal Biochem,1995,227(1):101-111．
【3】Bergh V,Vanhees I,De-Boer R,et al. Identification of the oxidation products of the reaction between α-pinene and hydroxyl radicals by gas and high-performance liquid chromatography with mass spectrometric detection[J]. J Chromatogr A,2000,896(1/2):135-148．
【4】任信荣,邵可声,唐孝炎.高效液相色谱法测定水杨酸及其羟基化产物[J].色谱,2001,19(2):191-192．
【5】Jen F J,Meei F L,Yang T C. Determination of hydroxyl radicals in an advanced oxidation process with salicylic acid trapping and liquid chromatography[J]. J Chromatogr A,1998,796(2):283-288．
【6】Coolen S A J,Huf F A,Reijenga J C. Determination of free radical reaction products and metabolites of salicylic acid using capillary electrophoresis and micellar electrokinetic chromatography[J]. J Chromatogr B,1998,717(1/2):119-126．
【7】Wang Q J,Ding F,Zhu N N,et al. Determination of hydroxyl radical by capillary zone electrophoresis with amperometric detection[J]. J Chromatogr A,2003,1016(1):123-128．
【8】Ai S Y,Wang Q J,Li H,et al. Study on production of free hydroxyl radical and its reaction with salicylic acid at lead dioxide electrode[J]. J Electroanal Chem,2005,578(2):223-229．
【9】陈义.毛细管电泳技术及应用[M].北京:化学工业出版社,2000:47．
【10】Halliwell B,Grootved M. Aromatic hydroxylation as a potential measure of hydroxyl-radical formation in vivo. Identification of hydroxylated derivatives of salicylate in human body fluids[J]. J Biochem,1986,237(2):499-504．
相关信息
