薄层色谱扫描法测定虎杖中大黄素的含量
Determination of Emodin in Giant Knotweed by Scanning Thin Layer Chromatography
摘 要
应用扫描薄层色谱法测定了中药虎杖中大黄素的含量,以硅胶作吸附剂的色层板作为固定相,以甲苯、乙酸乙酯及甲酸(体积比为30比4比2)的混合溶剂为展开剂.用双波长反射线性扫描对大黄素斑点进行定量检测,测定波长为440 nm,参比波长为600 nm.线性回归方程为y=1 899.985 0 x+122.946 1,相关系数为0.984 0,斑点峰面积的积分值与其中大黄素质量在0.40~0.81 μg之间呈线性关系.在同一硅胶板上,精密地点上相同量的对照标准溶液,每个点4 μL,作该方法的精密度试验,所得相对标准偏差(n=7)为0.95%.在含已知和不同量大黄素的试样中加入一定量的标准溶液作回收试验,所得结果在95.7%~99.4%之间.
大黄素
虎杖
Scanning thin layer chromatography
Emodin
Giant knotweed
Abstract
Scanning thin layer chromatography (TCL) was applied to the determination of emodin in giant knotweed,using silica gel as adsorbent supported on the TLC plate,and a mixture of toluene,ethylacetate and formic acid (mixed in the ratio of 30∶4∶2) as developing solvent. Quantitative measurement was made by DW reflective linear scanning of the emodin spot at the wavelengths of λS 440 nm and λR 600 nm. Linear relationship between values of integral of peak area of the spots and mass of emodin was obtained in the range of 0.40-0.81 μg,as shown be the linear regression equation of y=1 899.985 0 x+122.946 1 (r=0.984 0). Precision of the method was performed on the same silica gel plate by spotting 4 μL portions of reference standard solution of emodin of same content,and value of RSD′s (n=7) found was 0.95%. Test of recovery was made by adding standard solutions to samples with known contents of emodin,values of recovery found were in the range of 95.7%-99.4%.
中图分类号 O657.7
所属栏目 工作简报
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收稿日期 2007/4/8
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备注马稳(1968-),女,河南宁陵人,副教授,主要从事仪器分析方面的研究.
引用该论文: MA Wen,LI Dan. Determination of Emodin in Giant Knotweed by Scanning Thin Layer Chromatography[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(9): 878～879
马稳,李丹. 薄层色谱扫描法测定虎杖中大黄素的含量[J]. 理化检验－化学分册, 2008, 44(9): 878～879
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参考文献
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【3】李淑娟,沈丽霞,李 伟,等.大黄素药理作用研究进展[J].张家口医学院学报,2003,20(2):80-83．
【4】陈国军,陈然峰,苗长林,等.拉米夫定联合大黄素抗肝纤维化作用的临床研究[J].中国实验诊断学,2003,7(1):43-45．
【5】包国荣,陈丹,罗志毅.康欣口服液中大黄素的HPLC分析[J].福建分析测试,2004:13(1):1913-1915．
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【7】马稳,段香芝,李丹.薄层扫描法测定延胡索中延胡索乙素含量[J].信阳师范学院学报,2004,17(4):420-421．
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