流动注射化学发光法测定哈西奈德
FI-Chemiluminescence Determination of Halcinonede
摘 要
试验发现,肾上皮质激素药物哈西奈德对鲁米诺与N-溴代丁二酰亚胺(BSMD)在碳酸钠-碳酸氢钠缓冲介质中反应所产生的化学发光(CL)有明显增强作用,且其增强程度与此药物的质量浓度在1.0×10-6~1.0×10-4g·L-1之间呈线性关系,检出限(3S/N)为3×10-7g·L-1.根据上述结果并结合应用流动注射技术,提出了测定哈西奈德的流动注射化学发光方法.在浓度水平为4.0×10-5g·L-1条件下,按方法作11次平行试验,求得其相对标准偏差为3.0%.应用此法分析了3个哈西奈德药物的实样,并用药典方法进行对照试验.结果表明:两方法所得结果相符.
流动注射技术
N-溴代丁二酰亚胺
鲁米诺
哈西奈德
Chemiluminescence
Flow injection technique
N-bromosuccinimide
Luminol
Halcinonede
Abstract
The chemiluminescence (CL) produced by the reaction between luminol and N-bromosuccinimide (BSMD) in Na2CO3-NaHCO3 buffer medium was found to be strongly enhanced by the presence of halcinonede,an adrenocorticomimetic drug,and the magnitude of increase in CL-intensity was found to keep linear relationship with the concentration of halcinonede in the range of 1.0×10-6-1.0×10-4g·L-1,with a detection limit (3S/N) of 3×10-7g·L-1.Based on these facts and by applying the flow injection technique,a method of FI-CL determination of halcinonede in its preparation was proposed.Precision of the method was tested at the concentration level of 4.0×10-5g·L-1 by 11 determinations,and the value of RSD obtained was 3.0%.In analyzing 3 substantial samples of the drug,results obtained by this method were in consistency with the results found by the method of pharmacopoeia.
中图分类号 O657.31
所属栏目 工作简报
基金项目
收稿日期 2007/4/11
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备注白浩(1980-),男,河南人,硕士研究生,研究方向为化学发光分析.
引用该论文: BAI Hao,Lv Jiu-ru. FI-Chemiluminescence Determination of Halcinonede[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(10): 927～929
白浩,吕九如. 流动注射化学发光法测定哈西奈德[J]. 理化检验－化学分册, 2008, 44(10): 927～929
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参考文献
【1】马洁.乐肤液的制备及分光光度法测定[J].现代应用医学,1996,13(2):59．
【2】刘义庆,彭子琼,胡少华.哈西奈德的荧光分析法研究[J].江汉大学学报:自然科学版,2006,34(1):76．
【3】孙悦,安彦,左文坚.RP-HPLC法测定哈西奈德涂膜剂的含量及其原料的有关物质[J].药物分析杂志,2005,25(3):349．
【4】Safavi A,Karimi M A.Flow injection determination of cationic surfactants by using N-bromosuccinimide and N-chlorosuccinimide as new oxidizing agents for luminol chemiluminescence[J].Anal Chim Acta,2002,468(1):53．
【5】Wang Zhouping,Zhang Zhujun,Fu Zhifeng,et al.Flow-injection chemiluminescence determination of aminomethylbenzoic acid and aminophyline based on N-bromosuccinimide-luminol reaction[J].Talanta,2004,62(3):611．
【6】Safavi A,Karimi A,Nezhad R H.Flow injection determination of isoniazid using N-bromosuccinimide and N-chlorosuccinimide-luminol chemiluminescence systems[J].J Pharm Biomed Anal,2003,30(5):1499．
【7】方卢秋.NBSM-荧光素体系流动注射化学发光法测定盐酸二甲双胍[J].理化检验-化学分册,2003,39(9):512．
【2】刘义庆,彭子琼,胡少华.哈西奈德的荧光分析法研究[J].江汉大学学报:自然科学版,2006,34(1):76．
【3】孙悦,安彦,左文坚.RP-HPLC法测定哈西奈德涂膜剂的含量及其原料的有关物质[J].药物分析杂志,2005,25(3):349．
【4】Safavi A,Karimi M A.Flow injection determination of cationic surfactants by using N-bromosuccinimide and N-chlorosuccinimide as new oxidizing agents for luminol chemiluminescence[J].Anal Chim Acta,2002,468(1):53．
【5】Wang Zhouping,Zhang Zhujun,Fu Zhifeng,et al.Flow-injection chemiluminescence determination of aminomethylbenzoic acid and aminophyline based on N-bromosuccinimide-luminol reaction[J].Talanta,2004,62(3):611．
【6】Safavi A,Karimi A,Nezhad R H.Flow injection determination of isoniazid using N-bromosuccinimide and N-chlorosuccinimide-luminol chemiluminescence systems[J].J Pharm Biomed Anal,2003,30(5):1499．
【7】方卢秋.NBSM-荧光素体系流动注射化学发光法测定盐酸二甲双胍[J].理化检验-化学分册,2003,39(9):512．
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