毛细管柱气相色谱法测定银杏叶提取物中银杏萜内酯含量
GC Determination of Terpene Lactones in Gingko Leaf Extracts with Capillary Chromatographic Column
摘 要
对银杏叶提取物中银杏萜内酯(包括BB和GA,GB,GC,GJ)的毛细管柱气相色谱分离条件作了试验,达到银杏萜内酯成分的良好分离.选择了内标物角鲨烷,理论计算并直接测定了银杏萜内酯的相对质量校正因子.采用内标法测定了各组分的含量.BB、GA、GB、GC回收率依次为95.2%,95.5%,94.6%,96.1%.
银杏叶
银杏萜内酯
内标法
Capillary column-gas chromatography
Gingko leaves
Terpenes
Internal standard
Abstract
Chromatographic conditions for separation of terpenes (named in abbreviation as GA,GB,GC,GJ and BB) from extracts of gingko leaves with capillary column were studied,and the terpenes were satisfactorily separated.Squalane was used as internal standard in the determination.The relative mass correction factors were calculated theoratically and also determined experimentally.Tests for recovery of BB,GA,GB and GC were made by addition of standards to a sample of known terpene contents and analyzed by the proposed method,values of recovery obtained were 95.2%,95.5%,94.6% and 96.1% respectively.
中图分类号 O657.37
所属栏目 工作简报
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收稿日期 2007/5/17
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备注刘红梅(1971-),女,河北霸州人,高级实验师,主要从事化工产品应用研究与分析.
引用该论文: LIU Hong-mei,ZHOU Qing-xia,YANG Wen-ling. GC Determination of Terpene Lactones in Gingko Leaf Extracts with Capillary Chromatographic Column[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(10): 982～985
刘红梅,周庆霞,杨文玲. 毛细管柱气相色谱法测定银杏叶提取物中银杏萜内酯含量[J]. 理化检验－化学分册, 2008, 44(10): 982～985
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参考文献
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【2】Valrie Flesch,Monique Jacques.Relative importance of growthand light level on terpene content of ginkgo biloba[J].Phytochemistry,1992,31:1941-1945．
【3】池静端,刘爱茹,徐 礼.HPLC法测定银杏叶中3种内酯成分[J].药物分析杂志,1998,18(6):367-369．
【4】张鉴,潘见,谢惠明,等.反相高效液相色谱法测定银杏叶提取物中5种银杏内酯的含量[J].分析化学,2000,28(1):53-56．
【5】卢定强,欧阳平凯.反相高效液相色谱法测定银杏叶中银杏萜内酯的含量[J].药物分析杂志,2002,22(1):70-72．
【6】Van Beek T A,Taylor L T.Sample preparation of standardized extracts of ginkgo biloba by supercritical fluid extraction[J].Phytochemical Analysis,1996,7:185-191．
【7】Fabrizio F,Camponovo.Evaporative light scattering and thermospray mass spectrometry: two alternative methods for detection and quantitative liquid chromatographic determination of ginkgolides and bilobalide in ginkgo biloba leaf extracts and phytopharmaceuticals[J].Phytochemical Analysis,1995(6):141-148．
【8】J Thomposon B Strode III,Larry T Taylor,Teris A Van Beek.Supercritical fluid chromatography of ginkgolides A,B,C,and J and bilobalide[J].J Chromatogr A,1996,738:115-122．
【9】Terise A Van Beek,Gerrit P Lelyveld.Quantitation of bilobalide and ginkgolides A,B,C,and J by means of nuclear magnetic resonance spectroscopy[J].Phytochemical Analysis,1993,4:261-268．
【10】唐红芳,郑自强,朱晓雨,等.银杏叶中银杏萜内酯的GC-MS定性定量分析[J].中草药,2003,34(3):214-217．
【11】冷平生,王天华,吴京科,等.银杏萜内酯的提取和气相色谱与质谱分析[J].北京林业大学学报,2000,22(5):19-22．
【12】孙传经.毛细管气相色谱法[M].北京:化学工业出版社,1990:217-218．
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