Photometric Determination of Serum Albumen Based on Its Reaction with the Coordination Complex of Lead(Ⅱ) and Xylenol Orange
摘 要
在pH 6.1的磷酸氢二钠-柠檬酸钠-磷酸缓冲溶液中,血清蛋白与铅-二甲酚橙反应生成复合物,最大吸收波长为300 nm,以牛血清蛋白为分析物,当牛血清蛋白的质量浓度在7 mg·L-1以内遵守比耳定律,其检出限(3S/N)为7.4×10-3mg·L-1.应用所提出的方法分析了3组人血清蛋白试样,所得结果用常规的CBBG-250方法作了校核.
Abstract
In a buffer solution of disodium hydrogenphosphate,sodium citrate and phosphoric acid of pH 6.1,a compound complex was formed between serum alumumen and the complex of Pb2+ with xylenol orange (XO),having its absorption maximum at 300 nm.Taking bovine serum albumien (BSA) as analyte,beer′s law was obeyed within 7 mg·L-1,with a detection limit of 7.4×10-3mg·L-1.The proposed method was used in analysis of 3 human serum albumen samples,and the results obtained were checked by the conventional method of CBBG-250.
中图分类号 O657.32
所属栏目 工作简报
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收稿日期 2007/5/11
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备注朱化雨(1962-),男,山东费县人,教授,主要从事分析化学的教学和研究工作,研究方向为现代分离技术和环境分析.
引用该论文: ZHU Hua-yu,LIU Gui-ming,ZHANG Yuan,SONG Xing-liang. Photometric Determination of Serum Albumen Based on Its Reaction with the Coordination Complex of Lead(Ⅱ) and Xylenol Orange[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(10): 989~990
朱化雨,刘桂明,张援,宋兴良. 基于铅(Ⅱ)-二甲酚橙配合物反应光度法测定血清蛋白[J]. 理化检验-化学分册, 2008, 44(10): 989~990
被引情况:
【1】蒋爱雯,段艳青,袁维国,王修中, "荧光光谱法研究间硝基苯胺与牛血清白蛋白的相互作用",理化检验-化学分册 51, 200-204(2015)
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参考文献
【1】罗宗铭,张艰,崔英德,等.蛋白质与酸性染料相互作用的分光光度研究[J].光谱学与光谱分析,2001,21(2):251.
【2】Hu quluan,Zhao Bangtun,Shen Yiyang.The study on the determination of protein based on the interaction between protein and complex of Cu(Ⅱ)-Arsenazo K[J].Qlerracal journal on Internet,2001,3(6):23-27.
【3】Brandford M M.A rapid and sensitive method for the ruantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Anal Biochem,1976,72(12):248-254.
【4】曹稳根,焦庆才,刘茜,等.考马斯亮蓝显色剂变色反应机理的研究[J].化学学报,2002,60(9):1656-1661.
【5】高峰,朱昌青.灿烂甲酚蓝与表面活性剂的作用及其在蛋白质测定中的应用[J].分析化学,2002,30(3):324-326.
【6】吴诚.机械工程材料测试手册[M].沈阳:辽宁科学技术出版社,1996:480.
【7】陶慰孙.蛋白质分子基础[M].北京:高等教育出版社,1885:243.
【2】Hu quluan,Zhao Bangtun,Shen Yiyang.The study on the determination of protein based on the interaction between protein and complex of Cu(Ⅱ)-Arsenazo K[J].Qlerracal journal on Internet,2001,3(6):23-27.
【3】Brandford M M.A rapid and sensitive method for the ruantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Anal Biochem,1976,72(12):248-254.
【4】曹稳根,焦庆才,刘茜,等.考马斯亮蓝显色剂变色反应机理的研究[J].化学学报,2002,60(9):1656-1661.
【5】高峰,朱昌青.灿烂甲酚蓝与表面活性剂的作用及其在蛋白质测定中的应用[J].分析化学,2002,30(3):324-326.
【6】吴诚.机械工程材料测试手册[M].沈阳:辽宁科学技术出版社,1996:480.
【7】陶慰孙.蛋白质分子基础[M].北京:高等教育出版社,1885:243.
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