高效液相色谱法测定苹果与山楂制品中展青霉素
Determination of Patulin in Apple and Hawthorn Products by High Performance Liquid Chromatography
摘 要
利用高效液相色谱法测定了苹果、山楂等制品中的展青霉素.样品经乙酸乙酯提取、碳酸钠净化后进行检测,通过对流动相、检测波长等试验条件的优化,消除了5-羟甲基糠醛对展青霉素测定的干扰,方法的回收率为95.2%~107.1%;检出限(3S/N)为0.04 mg·L-1,线性范围在0.14~2.8 mg·L-1.
高效液相色谱
5-羟甲基糠醛
Patulin
High performance liquid chromatography
5-Hydroxymethylfurfural
Abstract
An HPLC method for the determination of patulin in apple and hawthorn products was proposed and reported in this paper.Patulin in sample was extracted by ethyl acetate,the extract was purified with Na2CO3 and followed by HPLC determination.Composition of eluant and wavelength for detection wavelength were tested and optimized so that interferences of 5-hydroxymethylfurfural (HMF) was eliminated.Recovery of the method was in range of 95.2%-107.1%.Detection limit (3S/N) was 0.04 mg·L-1 and linearity of calibration curve was ranged in 0.14-2.8 mg·L-1 of patulin.
中图分类号 O657.57
所属栏目 工作简报
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收稿日期 2007/5/13
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备注徐春祥(1970-),男,南京人,高级工程师,主要从事食品、化妆品分析检验工作.
引用该论文: XU Chun-xiang,YE Qiang,WU Yi-bing,JIANG Mei. Determination of Patulin in Apple and Hawthorn Products by High Performance Liquid Chromatography[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(10): 991～993
徐春祥,叶强,吴奕兵,姜梅. 高效液相色谱法测定苹果与山楂制品中展青霉素[J]. 理化检验－化学分册, 2008, 44(10): 991～993
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参考文献
【1】宋家玉,邢金川,林艺,等.山东省部分水果及制品中展青霉素污染调查分析[J].中国食品卫生杂志,1995,7(2):43-44．
【2】刘绘园,于孔玖.山楂及其制品中展青霉素的含量及污染的调查分析[J].中国食品卫生杂志,1995,7(4):42．
【3】AOAC Official Method 995.10Patulin in Apple Juices Liquid Chromatographic Method[S]．
【4】祁广建,沈国英,李晓雯,等.高效液相色谱测定水果制品中展青霉素的含量[J].中国公共卫生,1999,15(2):145-146．
【5】黄伟,余以刚,刘继平,等.水果及果汁饮料中展青霉素和HMF分析方法研究进展[J].中国卫生检验杂志,2003,1(13):119-120．
【6】GB/T 5009.185-2003苹果和山楂制品中展青霉素的测定[S]．
【2】刘绘园,于孔玖.山楂及其制品中展青霉素的含量及污染的调查分析[J].中国食品卫生杂志,1995,7(4):42．
【3】AOAC Official Method 995.10Patulin in Apple Juices Liquid Chromatographic Method[S]．
【4】祁广建,沈国英,李晓雯,等.高效液相色谱测定水果制品中展青霉素的含量[J].中国公共卫生,1999,15(2):145-146．
【5】黄伟,余以刚,刘继平,等.水果及果汁饮料中展青霉素和HMF分析方法研究进展[J].中国卫生检验杂志,2003,1(13):119-120．
【6】GB/T 5009.185-2003苹果和山楂制品中展青霉素的测定[S]．
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