试验发现由氢溴酸右美沙芬(D-MPHB)与高锰酸钾在5.0 mmol·L-1硫酸介质中反应所产生的较弱的化学发光强度因一定量的亚硫酸钠的存在而明显增强,且其化学发光的相对强度与D-MPHB浓度在1.0×10-5~1.0×10-2g·L-1范围内呈线性关系,检出限(3S/N)为3.5×10-6g·L-1.根据这些结果并应用流动注射技术,提出了FI-CL法测定药物中氢溴酸右美沙芬的方法.应用所提出的方法分析了一种氢溴酸右美沙芬滴鼻液,测得其中D-MPHB的含量与标准值相符.以此药物为基体对方法的精密度及回收率作了试验,测得其相对标准偏差(n=5)均小于1.5%,回收率在99.7%~102.8%之间.

It was found that the intensity of weak chemiluminescence (CL) produced by the reaction of dextromethorphan hydrobromide (D-MPHB) with KMnO4 in 5.0 mmol·L-1 H2SO4 medium was strongly enhanced in the presence of appropriate amount of Na2SO3,and linear relationship between the magnitude of relative CL intensity and concentration of D-MPHB was obtained in the range of 1.0×10-5-1.0×10-2g·L-1 with a detection limit (3S/N) of 3.5×10-6g·L-1.Base on these facts and by using the flow injection technique,a new method of FI-chemiluminescence determination of D-MPHB in its preparations was proposed.In its application to the analysis of a sample of dextromethrophan hydrobromide nasal drops,the contents of D-MPHB found were in consistency with the labelled value,and tests for precision and recovery were made on the base of this sample,giving values of RSD′s (n=5) less than 1.5% and of recovery in the range of 99.7%-102.8%.