Assay of Borneol by the Color Reaction with Vanillin
摘 要
冰片样品及纯冰片标准样用无水乙醇为溶剂,利用香草醛在浓硫酸中与龙脑及异龙脑的显色反应,用分光光度法测定冰片含量,测定波长为470 nm.在优化条件下,吸光度与龙脑浓度之间在0.06~0.30 g·L-1范围内呈线性关系,对应于线性回归方程A=2.850 1 C+0.015 6,相关系数为0.999 3;测得三批冰片样品中龙脑质量浓度分别为0.093,0.097,0.096 g·L-1.在同一试样中加入3种不同浓度的龙脑标准溶液按试验方法进行回收试验,测得回收率在93.3%~98.5%之间.
Abstract
Borneol sample or the reference standard of pure borneol was dissolved in absolute alcohol.Through the reaction with vanillin in conc.H2SO4,a rose colored compound was formed by borneol and its isomer,having its absorption maximum at the wavelength of 470 nm.Under optimum conditions,linear relationship between values of absorbance and concentration of borneol was obtained in the range of 0.06-0.30 g·L-1,in respective to the linear regression equation A=2.850 1 C+0.015 6 (r=0.999 3),c in the equation representing the concentration of borneol in g·L-1.Samples from 3 batches of borneol were analyzed by this methods,giving results of total mass concentration of borneol content 0.093,0.097,0.096 g·L-1 respectively.Recovery tests were made by adding different amounts of standard borneol solution to a borneol sample,and values of recovery obtained were from 93.3% to 98.5%.
中图分类号 O657.31
所属栏目 工作简报
基金项目 福建省教育厅科技计划资助项目(JA07101)
收稿日期 2007/5/23
修改稿日期
网络出版日期
作者单位点击查看
备注李光文(1963-),男,福州市人,高级实验师,主要研究方向为天然活性物质基础分析.
引用该论文: LI Guang-wen,SUN Yuan-yuan,YAO Hong. Assay of Borneol by the Color Reaction with Vanillin[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(11): 1083~1084
李光文,孙媛媛,姚宏. 通过与香草醛的显色反应检测冰片成分[J]. 理化检验-化学分册, 2008, 44(11): 1083~1084
共有人对该论文发表了看法,其中:
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】冯明霞,王桂兰,闻京伟.气相色谱法测定除疤膏中冰片的含量[J].中国药事,2005,19(12):743.
【2】范蓉,冯明霞.GC测定舒肤止痒酊中冰片的含量[J].中成药,2006,28(1):142.
【2】范蓉,冯明霞.GC测定舒肤止痒酊中冰片的含量[J].中成药,2006,28(1):142.
相关信息