水样中7种酚同系物用固相萃取法在Oasis HLB柱上预富集,经吹氮干燥后用1 mL甲醇将包括苯酚、4-硝基酚、3-甲基酚、2-氯酚、2,4-二氯酚、2,4,6-三氯酚及五氯酚在内的7种酚同系物从柱上淋洗,所得淋出液直接进样进行高效液相色谱法测定.在所提出的最佳测定条件下,4-硝基酚质量浓度在4.0 mg·L-1以内,其余6种酚同系物质量浓度在20.0 mg·L-1以内与相应的检测信号之间呈线性关系,以10倍信噪比计算方法的测定下限,测得4-硝基酚的测定下限为0.02 mg·L-1,其余6种酚同系物为0.1 mg·L-1以下.用标准加入法测定方法的回收率,所用基体水样中未检出上述7种酚同系物,测得回收率在79.4%~103.0%之间,相应的相对标准偏差(n=7)在1.0%~6.7%之间.

Seven phenol homologues in water sample were enriched by solid phase extraction (SPE) on the Oasis HLB column.After drying by N2-blowing,phenols (including phenol,4-nitrophenol,3-methyl phenol,2-chlorophenol,2,4-dichlorophenol,2,4,6-trichlorophenol,and pent chlorophenol) were eluted from the column with 1 mL of methanol,which was introduced into the instrument and determined by HPLC.Under the optimum condition of determination,linear relationships between values of analytical signals and concentration of the phenols were obtained in the range within 4.0 mg·L-1 for 4-nitrophenol,and in ranges within 20.0 mg·L-1 for the remainders.Lower limits of determination (10S/N) found for 4-nitrophenol was 0.02 mg·L-1,and for the remainder were less than 0.1 mg·L-1.Tests for recovery of the method were made by addition of standard solutions of the 7 phenol homologues to water samples in which phenols were not detected,values of recovery obtained were in the range of 79.4% to 103.0%,with values of RSD′s (n=7) in the range of 1.0%-6.7%.