高效凝胶渗透色谱及高效液相色谱和电喷雾-离子阱质谱法联用测定构成芦荟多糖的单糖
Determination of the Compositional Monosaccharides of Polysaccharides in Aloe by HPGPC,HPLC and ESI-MS
摘 要
利用高效凝胶渗透色谱(HPGPC)测定芦荟浓缩液和芦荟粉中多糖的纯度和相对分子质量,使用1-苯基-3-甲基-5-吡唑啉酮(PMP)作为柱前衍生试剂分别对样品和其水解产物进行衍生,结合高效液相色谱(HPLC)分离和电喷雾-离子阱质谱(ESI-MS)检测分析其单糖组成.结果表明:组成芦荟中多糖的单糖主要是甘露糖和葡萄糖.
单糖
高效凝胶渗透色谱
高效液相色谱
电喷雾-离子阱质谱
柱前衍生
1-苯基-3-甲基-5-吡唑啉酮
Aloe polysaccharide
Monosaccharides
High performance gel permeation chromatography (HPGPC)
High-performance liquid chromatography (HPLC)
Electron spray ionization-mass spectroscopy (ESI-MS)
Precolumn derivatization
1-Phenyl-3-methyl-5-pyrazolone
Abstract
High performance gel permeation chromatography (HPGPC) was applied to the determination of purity and relative molecular mass of polysaccharides in concentrate liquor and powder of aloe.The aloe sample (in liquor or powder state) or its hydrolysis product was pre-column derivatized with 1-phenyl-3-methyl-5-pyrazolone (PMP),and the compositional monosaccharides were separated and determined by hyphenation of HPLC with electron spray ionization-mass spectroscopy (ESI-MS).It was found that mannose and glucose were the main compositional monosaccharides of polysaccharides in aloe.
中图分类号 O657.7
所属栏目 试验与研究
基金项目
收稿日期 2008/3/24
修改稿日期
网络出版日期
作者单位点击查看
备注徐瑾(1978-),女,常州人,讲师,硕士研究生,主要研究液相色谱在食品、药品、化学品分析中的应用.
引用该论文: XU Jin. Determination of the Compositional Monosaccharides of Polysaccharides in Aloe by HPGPC,HPLC and ESI-MS[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(12): 1133～1136
徐瑾. 高效凝胶渗透色谱及高效液相色谱和电喷雾-离子阱质谱法联用测定构成芦荟多糖的单糖[J]. 理化检验－化学分册, 2008, 44(12): 1133～1136
共有人对该论文发表了看法，其中：
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】方积年.多糖研究的现状[J].药学学报,1986,21(12):944-950．
【2】方积年.多糖的分离纯化及其纯度鉴别与分子量测定[J].药学通报,1984,19:46-50．
【3】魏远安,方积年.高效凝胶渗透色谱法测定多糖纯度及分子量[J].药学学报,1989,24(7):532-536．
【4】成跃祖.凝胶渗透色谱法的进展及其应用[M].北京:中国石化出版社,1993:10-78．
【5】Schwald W,Concin R,Bonn Obobleter G.Analysis of oligomeric and monomeric carbohydrates from hydrothermal degradation of cotton-waste materials using HPLC and GPC[J].Chromatographia,1985,20(1):35-40．
【6】Praznik W,Beck R H F.New high-performance gel permeation chromatographic system for the determination of low-molecular-weigh amyloses[J].J of Chromatogr,1987,387:467-472．
【7】Lecacheux D,Panaras R,Prechner R,et al.Fractionation of natural polysaccharides by preparative GPC[J].Spectra,2000,1987,122:61-62．
【8】梁振,徐瑾,张维冰,等.啤酒中单糖的衍生化HPLC-ESI-MS测定方法研究[J].分析试验室,2004,23(5):27-30．
【9】Mabusela Wilfred T,Stephen Alistair M,Botha Marthinus C.Carbohydrate polymers from aloe ferox leaves[J].Phytochemistry,1990,29(11):3555-3558．
【2】方积年.多糖的分离纯化及其纯度鉴别与分子量测定[J].药学通报,1984,19:46-50．
【3】魏远安,方积年.高效凝胶渗透色谱法测定多糖纯度及分子量[J].药学学报,1989,24(7):532-536．
【4】成跃祖.凝胶渗透色谱法的进展及其应用[M].北京:中国石化出版社,1993:10-78．
【5】Schwald W,Concin R,Bonn Obobleter G.Analysis of oligomeric and monomeric carbohydrates from hydrothermal degradation of cotton-waste materials using HPLC and GPC[J].Chromatographia,1985,20(1):35-40．
【6】Praznik W,Beck R H F.New high-performance gel permeation chromatographic system for the determination of low-molecular-weigh amyloses[J].J of Chromatogr,1987,387:467-472．
【7】Lecacheux D,Panaras R,Prechner R,et al.Fractionation of natural polysaccharides by preparative GPC[J].Spectra,2000,1987,122:61-62．
【8】梁振,徐瑾,张维冰,等.啤酒中单糖的衍生化HPLC-ESI-MS测定方法研究[J].分析试验室,2004,23(5):27-30．
【9】Mabusela Wilfred T,Stephen Alistair M,Botha Marthinus C.Carbohydrate polymers from aloe ferox leaves[J].Phytochemistry,1990,29(11):3555-3558．
相关信息
