在pH 3.06的BR缓冲介质中,链霉素(SM)与滂胺天蓝(PSB)反应生成离子缔合物,出现了吸收峰在622 nm波长处的负吸收光谱,与PSB的正吸收峰(612 nm)相比,红移了10 nm,在622 nm波长处吸光度负值(-A)的增加与SM浓度之间在0.17×10-7~1.8×10-5mol·L-1范围内呈线性关系,其表观摩尔吸光率(ε622)为1.06×104L·mol-1·cm-1.应用此方法分析了3瓶链霉素硫酸盐注射液样品,测得其SM含量与标示值相符.在注射液样品的基础上加入SM标准溶液作回收试验,测得回收率在99.1%~101.3%之间.

In a BR buffer medium of pH 3.06,an association complex was formed between streptomycin (SM) and pontamine sky blue (PSB),leading to appearance of a negative absorption spectrum with its absorption maximum at the wavelength of 622 nm,bathochromically shifted 10 nm from the positive absorption maxmum (612 nm) of PSB.Linear relationship between the values of increase in negative absorbance (-A) and concentration of SM was obtained in the range of 0.17×10-7-1.8×10-5mol·L-1 with the apparent molar absorptivity (ε622) of 1.06×104L·mol-1·cm-1.The proposed method was applied to the analysis of 3 samples of streptomycin sulfate injection,giving values of SM contents in consistency with the labelled values.Test for recovery was performed by adding standard SM solution to the injection samples,and values of recovery found were in the range from 99.1% to 101.3%.