Capillary Column-Gas Chromatographic Determination of Camphor,Menthol,Borneol and Isoborneol in Plaster of Traditional Chinese Medicines Using a Modified Soxhlet′s Extractor
摘 要
用毛细管柱气相色谱法测定了中药膏药中樟脑、薄荷脑、龙脑及异龙脑的含量.应用一种改进型的索氏提取器和丙酮作为溶剂从膏药样品中提取上述4种组分.试验结果显示:此种改进的提取器的提取效率高于未改进的提取器约40%,从丙酮提取液中分取一定量的试液进行气相色谱测定,用纯萘作内标,测得上述4种化合物的线性范围依次为21.28~1.915×103,26.02~2.342×103,7.9~7.11×102,4.42~397.8 mg·L-1,分别做了回收试验和精密度试验,测得回收率在92.1%~99.5%之间,相对标准偏差(n=7)在1.1%~3.5%之间.
Abstract
A modified Soxhlet′s extractor was used in the capillary column gas-chromatographic determination of camphor,menthol,borneol and iso-borneol in plasters of traditional Chinese medicines,for extraction of the above mentioned compounds.It was shown that the efficiency of extraction of the modified Soxhlet′s extractor was raised about 40% as compared with the conventional model.Acetone was used as the extraction solvent.An aliquot of the extract was used for GC determination using naphthalene as internal standard.Data of linearity ranges obtained were as follows: ① for camphor: 21.28-1.915×103mg·L-1;② for menthol: 26.02-2.342×103mg·L-1;③ for borneol 7.9-7.11×102mg·L-1 and ④ for isoborneol: 4.42-397.8 mg·L-1.Tests for recovery and precision of the method using the modified extractor was performed,giving values of recovery in the range of 92.1%-99.5%,and values of RSD′s (n=7) in the range from 1.1% to 3.5%.
中图分类号 O657.8
所属栏目 工作简报
基金项目
收稿日期 2007/6/25
修改稿日期
网络出版日期
作者单位点击查看
备注阎正(1958-)男,河北保定人,副教授,主要从事色谱分析技术研究.
引用该论文: YAN Zheng,CHEN Pei-yun,CAO Zhao-zhen,YAN Hai-rong. Capillary Column-Gas Chromatographic Determination of Camphor,Menthol,Borneol and Isoborneol in Plaster of Traditional Chinese Medicines Using a Modified Soxhlet′s Extractor[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(12): 1196~1198
阎正,陈培云,曹照真,阎海荣. 毛细管柱-气相色谱法测定中药膏药中樟脑、薄荷脑、龙脑及异龙脑含量-应用一种改进型索氏提取器[J]. 理化检验-化学分册, 2008, 44(12): 1196~1198
共有人对该论文发表了看法,其中:
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】阎正,刘鹏岩,张建申,等.气相色谱法测定冠心膏中樟脑、薄荷脑、异龙脑、龙脑含量[J].色谱,1998,16(5):411-413.
【2】阎正,刘树彬,葛旭升,等.毛细管气相色谱法快速测定中成药橡胶膏剂中樟脑、薄荷脑、冰片含量[J].河北大学学报,2001,21(3):291-295.
【3】徐小平,宋玉如.毛细管气相色谱法测定冰片-B-环糊精包结物中冰片的含量[J].华西药学杂志,1989,4(1):55-56.
【4】伍朝筼,蒋学华,马鹏,等.毛细管气相色谱法测定冰片及含冰片中成药中龙脑和异龙脑的含量[J].华西药学杂志,1989,4(1):9-12.
【2】阎正,刘树彬,葛旭升,等.毛细管气相色谱法快速测定中成药橡胶膏剂中樟脑、薄荷脑、冰片含量[J].河北大学学报,2001,21(3):291-295.
【3】徐小平,宋玉如.毛细管气相色谱法测定冰片-B-环糊精包结物中冰片的含量[J].华西药学杂志,1989,4(1):55-56.
【4】伍朝筼,蒋学华,马鹏,等.毛细管气相色谱法测定冰片及含冰片中成药中龙脑和异龙脑的含量[J].华西药学杂志,1989,4(1):9-12.
相关信息