离子色谱法同时测定牛瘤胃液中乳酸和挥发性脂肪酸含量
Simultaneous Determination of Lactic Acid and Volatil Fatty Acids in Ox Rumen Juice by Ion Chromatography
摘 要
提出了同时测定牛瘤胃液中乳酸和挥发脂肪酸的离子色谱分析法.试验用IonPac AS11-HC(4 mm×250 mm)分析柱带IonPac AG11-HC(4 mm×50 mm)保护柱,电导检测器,抑制器类型为ASRS.以不同浓度的氢氧化钠溶液为流动相,梯度洗脱.该方法对乳酸、乙酸、丙酸、异丁酸、丁酸、异戊酸、戊酸的线性范围分别为0.36~36.0,0.10~50.0,0.11~23.0,0.05~8.7,0.15~36.0,0.11~19.0,0.06~23.5 mg·L-1,方法检出限(3S/N)依次为9.87,3.60,4.15,2.46,4.71,3.19,6.93 μg·L-1.实样分析结果的相对标准偏差(n=7)在1.73%~5.66%之间.
瘤胃液
乳酸
挥发脂肪酸
Ion chromatography
Lactic acid
Volatile fatty acids
Ox rumen juice
Abstract
Ion chromatography was applied to the simultaneous determination of 7 organic acids in rumen juice,including lactic acid,ethanoic acid,propanoic acid,iso-butanoic acid,n-butanoic acid,iso-pentanoic acid and n-pentanoic acid.The IonPac AS11-HC (4 mm×250 mm) was used as analytical column with IonPac AG11-HC (4 mm×50 mm) as protective column.Electric conductance detector with ASRS (4 mm) suppressor was used in the determinations.Sodium hydroxide solutions with different concentration were used as the mobile phase in the gradient elution.Linear relationships between the values of peak area and concentration of the above mentioned organic aicds were found in ranges of 0.36-36.0,0.10-50.0,0.11-23.0,0.05-8.7,0.15-36.0,0.11-19.0 and 0.06-23.5 mg·L-1 with their detection limits of 9.87,3.60,4.15,2.46,4.71,3.19,6.93 μg·L-1 respectively.As shown by the analytical results obtained by analysis of substantial sample of rumen juice,values of RSD′s (n=7) found from the analytical results were in the range of 1.73%-5.66%.
中图分类号 O657.7
所属栏目 工作简报
基金项目 农业部948项目谷物饲料蒸汽压片处理关键设备和技术引进资助(2003-Z77)
收稿日期 2007/11/3
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备注牟豪杰(1981-),男,山东烟台人,硕士研究生,研究方向为仪器分析.
引用该论文: Mu Hao-jie,MENG Qing-xiang,REN Li-ping,WANG Li. Simultaneous Determination of Lactic Acid and Volatil Fatty Acids in Ox Rumen Juice by Ion Chromatography[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(1): 52～54
牟豪杰,孟庆翔,任丽萍,王利. 离子色谱法同时测定牛瘤胃液中乳酸和挥发性脂肪酸含量[J]. 理化检验－化学分册, 2009, 45(1): 52～54
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参考文献
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【3】苏国岁,苏红军.烟用香精中乳酸的快速气相色谱分析[J].现代仪器,2002,3:24-26．
【4】Mataix E,Luque de Castro M D.Determination of l-(-)-malic acid and l-(+)-lactic acid in wine by a flow injection-dialysis-enzymic derivatisation approach[J].Anal Chim Acta,2001,428:7-14．
【5】Güzel Seydim Z B,Seydim A C,Greene A K,et al.Determination of organic acids and volatile flavor substances in kefir during fermentation[J].Journal of Food Composition And Analysis,2000,13:35-43．
【6】Lourdes Morales M,Gustavo Gonzalez A,Troncoso Ana M.Ion-exclusion chromatographic determination of organic acids in vinegars[J].Journal of Chromatography A,1998,822:45-51．
【7】傅彤,刘庆生,范志影,等.应用离子色谱测定青贮饲料中有机酸含量的研究[J].中国畜牧兽医,2005,32(5):16-17．
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