采用毛细管柱气相色谱法测定油脂中7种脂肪酸甲酯的含量,选用HP-INNOWAX毛细管色谱柱(1 μm,30 m×0.53 mm),氢火焰离子化(FID)检测器,进样口温度250 ℃,柱温220 ℃,检测器温度280 ℃,载气流量128 mL·min-1的色谱条件,以保留时间定性,面积归一法定量.按所提的方法测定了花生油标样中7种脂肪酸甲酯的含量,测得结果与标准值相符合,测定值的相对标准偏差在0.1%~5.6%之间.

Capillary column gas chromatography was proposed for the determination of methyl esters of fatty acids in this paper.The capillary column HP-INNOWAX (1 μm,30 m×0.53 mm,polyethylene glycol) and FID detector were used in this work.Temperature conditions were selected as follows:for injector inlet 250 ℃,for column 220 ℃ and for detector 280 ℃.Flow rate of carrier gas nitrogen was 128 mL·min-1.Qualitative analysis was based on the retention time and the normalized peak area was used for quantitative analysis.The proposed method was applied to the determination of 7 methyl esters of fatty acids in standard sample of peanut oil,giving results in consistency with the standard values,and values of RSD′s obtained were in the range from 0.1% to 5.6%.