Simultaneous Determination of Protein and Fat in Milk Powder by FTIRS
摘 要
利用傅里叶变换红外光谱法同时测定奶粉中蛋白质和脂肪含量.以硫氰化钾为内标物,其特征峰2 061.8 cm-1为参比峰,蛋白质和脂肪的特征峰为分析峰,分别将蛋白质和脂肪的分析峰的校正面积(As)与参比峰的校正面积(AKSCN)比(R=As/AKSCN)对蛋白质和脂肪含量作工作曲线.确定酰胺Ⅰ带1 654.8 cm-1(1 600.0~1 733.3 cm-1)与参比峰(1 981.0~2 142.9 cm-1)的组合A1 654.8/AKSCN最适合进行蛋白质定量分析,其线性回归方程C=3.871 R+10.88,相关系数为0.992 3;脂肪的C=O伸缩振动1 747.4 cm-1(1 719.0~1 766.7 cm-1)与参比峰(1 985.7~2 133.3 cm-1)的组合A1 747/AKSCN最适合脂肪定量分析,其线性回归方程C=29.13 R+3.531,相关系数为0.986 6.此方法的测定结果与凯氏定氮法和碱性乙醚提取法测定结果相比相对误差均小于±2.4%.
Abstract
Fourier transform-infrared spectrometry (FTIRS) was applied to the rapid determination of protein and fat in milk powder using potassium thiocyanate as internal standard and its characteristic peak of 2 061.8 cm-1 as reference peak,and the characteristic peaks of protein and fat as analytical peaks.Working curves were prepared by plotting the ratio of corrected peak area of protein or fat (As) to that of potassium thiocyanate (ASCN) versus content of protein or fat.Ratio (R1) of peak area of the amide Ⅰ band of protein at 1 654.8 cm-1 (1 600.0-1 733.3 cm-1) and the peak area of reference peak (1 981.0-2 142.9 cm-1),A1 654.8/ASCN-,was taken as parameter in determination of protein,and the linear regression equation obtained was C1=3.871 R1+10.88 (r=0.992 3).Ratio (R2) of peak area of the stretching vibration band of C=O group of fat at 1 747.4 cm-1 to that of peak area of reference peak (1 985.7-2 133.3 cm-1),A1 747.4/ASCN-,was taken as parameter in determination of fat,and the linear regression equation obtained was C2=29.13 R2+3.531 (r=0.986 6).Values of relative error between results obtained by this method and by the kjeldahl′s method for protein or the ether extraction method for fat were all within the range ±2.4%.
中图分类号 O657.33
所属栏目 工作简报
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收稿日期 2008/1/3
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备注周永国(1964-),男,河北省秦皇岛人,教授,主要从事天然产物化学研究工作.
引用该论文: ZHOU Yong-guo,HOU Wen-long,YANG Yue-dong,ZHAO Yong-guang. Simultaneous Determination of Protein and Fat in Milk Powder by FTIRS[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(1): 66~69
周永国,侯文龙,杨越冬,赵永光. 傅里叶变换红外光谱法同时测定奶粉中蛋白质和脂肪含量[J]. 理化检验-化学分册, 2009, 45(1): 66~69
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参考文献
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【4】王丽杰,郭建英,徐可欣.对牛奶成分近红外光谱分析方法的研究[J].哈尔滨理工大学学报,2004,9(5):36-38.
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