气相色谱-质谱联用法测定烟用香精成分-烟草用香精前处理方法比较
GC-MS Determination of Flavour in Tobacoo-Comparative Study of Extraction Methods of Tobacoo Flavour
摘 要
采用同时蒸馏萃取(SDE)、超声辅助液液萃取(ULLE)和固相微萃取(SPME)3种前处理方法提取HMT-3烟用香精成分,用气相色谱-质谱联用法(GC-MS)进行分析.对3种萃取方法鉴定出的组分作出比较,考察了方法的各自特点.选取了15种烟用香精为代表,分别采用3种萃取方法对不同类型的烟用香精进行提取.结果表明:ULLE操作简便、快速、节能、萃取效率高;SDE法和ULLE法的重现性明显好于SPME法,适合于香精的定量分析,而SPME有较高的灵敏度,更适合香精的定性分析.根据定性和定量的需要、方法的适用性和香精的物理状态,应选取不同的提取方法,从而能够快速、准确地获得样品信息.
超声辅助液液萃取
固相微萃取
GC-MS
香精
烟草
Simultaneous distillation extraction
Ultrasound-assisted liquid-liquid extraction
Solid phase micro extraction
GC-MS
Flavour
Tobacoo
Abstract
Three methods of extraction of 15 flavour in tobacoo for GC-MS analysis were studied in this paper.Components extracted by respective method were compared.Experimental study demonstrated that ultrasound-assisted liquid-liquid extraction (ULLE) has high extraction efficiency and simple to operate,both simultaneous distillation extraction (SDE) and ULLE were better in repeatability than solid phase micro extraction (SPME) as that they were suitable for the quantitative determination of flavour and SPME was suitable for the qualitative detection of flavour because of its high sensitivity.Extraction method should be selected on the basis of the analytical purpose and the physical properties of the flavour so as to obtain accurate information of the flavour in tobacoo.
中图分类号 O657.7
所属栏目 工作简报
基金项目
收稿日期 2008/2/19
修改稿日期
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备注曲国福(1981-),男,辽宁抚顺人,硕士研究生,主要从事烟用香精香料研究.
引用该论文: QU Guo-fu,LU She-ming,MENG Zhao-yu,ZHAN Jia-fen,DING Zhong-tao,MIAO Ming-ming. GC-MS Determination of Flavour in Tobacoo-Comparative Study of Extraction Methods of Tobacoo Flavour[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(1): 79～81
曲国福,陆舍铭,孟昭宇,詹家芬,丁中涛,缪明明. 气相色谱-质谱联用法测定烟用香精成分-烟草用香精前处理方法比较[J]. 理化检验－化学分册, 2009, 45(1): 79～81
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参考文献
【1】朱书奎,路鑫,邢钧,等.全二维气相色谱/飞行时间质谱用于烟用香精化学组分的分析[J].分析化学,2006,34(2):191．
【2】郭方遒,钟科军,黄建国,等.烟用香精香气成分提取方法的选择[J].烟草科技,2005(12):20．
【3】Mircea Vinatoru.An overview of the ultrasonically assisted extraction of bioactive principles from herbs[J].Ultrasonics Sonochemistry,2001(8):303．
【4】惠康进,甘学文,陈晶波,等.烟用香精的气相色谱-质谱监控[J].烟草科技,2004(9):27．
【5】Peter Kusch,Gerd Knupp.Headspace-SPME-GC-MS Identification of Volatile Organic Compounds Released from Expanded Polystyrene[J].Journal of Polymers and the Environment,2004,12(2):83．
【2】郭方遒,钟科军,黄建国,等.烟用香精香气成分提取方法的选择[J].烟草科技,2005(12):20．
【3】Mircea Vinatoru.An overview of the ultrasonically assisted extraction of bioactive principles from herbs[J].Ultrasonics Sonochemistry,2001(8):303．
【4】惠康进,甘学文,陈晶波,等.烟用香精的气相色谱-质谱监控[J].烟草科技,2004(9):27．
【5】Peter Kusch,Gerd Knupp.Headspace-SPME-GC-MS Identification of Volatile Organic Compounds Released from Expanded Polystyrene[J].Journal of Polymers and the Environment,2004,12(2):83．
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