气相色谱法测定Fenton反应产生的羟基自由基及其清除作用
GC Determination of ·OH Free Radical and Its Scavenging Action
摘 要
提出了一种测定羟基自由基的气相色谱法.由Fenton反应产生的羟基自由基氧化二甲亚砜(DMSO),生成的甲醛经2,4-二硝基苯肼(DNPH)衍生化并用四氯化碳萃取.所得产物(甲醛-DNPH)利用HP-5毛细管柱进行分离,氢火焰离子化检测器检测,通过测定甲醛-DNPH的峰面积可间接定量羟基自由基的产生量.拟定的方法测定了几种羟基自由基清除剂的浓度.此法可用于羟基自由基清除剂的筛选.
清除剂
气相色谱法
Hydroxyl free radical
Scavenger
Gas chromatography
Abstract
A gas chromatography method for the determination of hydroxyl free radical was proposed.The hydroxyl free radical generated in Fenton Reaction was reacted with di-methyl sulfoxide (DMSO) to form formaldehyde,which was then derivatized with 2,4-dinitro-phenylhydrazine to form the corresponding hydrazone.The produced hydrazone (HCHO-DNPH) was extracted with CCl4,separated with HP-5 capillary column and determined by GC using FID as detector.Indirect quantification of the free radical was carried out by measuring the peak area of HCHO-DNPH.Scavenging action for hydroxyl free radical of several scavengers was determined.The method was feasible for sieving of scavengers for hydroxyl free radical.
中图分类号 O657.7
所属栏目 工作简报
基金项目 北京市教委基金(KM200510028006)
收稿日期 2008/2/14
修改稿日期
网络出版日期
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备注王炳娟(1983-),女,山西省晋城人,硕士研究生,主要研究方向为分离技术、电分析化学.
引用该论文: WANG Bing-juan,LU Qing,ZOU Hong. GC Determination of ·OH Free Radical and Its Scavenging Action[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(1): 88～90
王炳娟,卢青,邹洪. 气相色谱法测定Fenton反应产生的羟基自由基及其清除作用[J]. 理化检验－化学分册, 2009, 45(1): 88～90
被引情况:
【1】刘瑛,陈新,薛翠华, "考马斯亮蓝G250褪色光度法测定Fenton反应产生的羟基自由基",理化检验－化学分册 46, 475-477(2010)
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参考文献
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【2】Kilinc E.Determination of the hydroxyl radical by its adduct formation with phenol and liquid chromatography/electrochemical detection[J].Talanta,2005(65):876-881．
【3】杨春维,王栋.可见分光光度法检测环境模拟水相中Fenton反应产生的羟基自由基[J].环境技术,2005(1):29-31．
【4】Tang B,Zhang L,Geng Y.Determination of the antioxidant capacity of different food natural products with a new developed flow injection spetrofluorimetry detection hydroxyl radicals[J].Talanta,2005(65):769-775．
【5】Fukui S,Hanasaki Y,Ogawa S.High-performance liquid chromatographic determination of methanesul phinic acid as a method for the determination of hydroxyl radicals[J].Chromatogr,1993,630(1/2):187-193．
【6】Chao T,Jin F P,Jing F L,et al.Determination of hydroxyl radicals in advanced oxidation processes with dimethyl sulfoxide trapping and liquid chromatography[J].Anal Chim Acta,2004(527):73-80．
【7】Oliva-Teles M T,Paiga P,Delerue-Matos C M,et al.Determination of free formaldehyde in foundry resins as its 2,4-dinitrophenylhydrazone by liquid chromatography[J].Anal Chim Acta,2002,467:97-103．
【8】邰超,邹洪,谷学新,等.Fenton反应产生的羟基自由基及其清除的电化学方法测定[J].分析测试学报,2002,21(5):30-32．
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