锌试剂与牛血清白蛋白相互作用机理
On the Mechanism of the Interaction Between Zincon and Bovine Serum Albumin
摘 要
用可见光区分光光度法研究了锌试剂与牛血清白蛋白之间相互反应的机理,在pH 4.2的条件下,随着牛血清白蛋白浓度的逐渐增加,反应体系在锌试剂吸收峰465 nm波长处的吸光度逐渐降低而在新出现的两吸收峰564 nm及600 nm波长处的吸光度逐渐增加.据此得出结论,锌试剂与牛血清白蛋白相互反应生成了复合物,而此复合物的形成乃由于两反应物之间的静电吸引作用及相互之间的疏水作用,而以后者为主导作用.在锌试剂与牛血清白蛋白的反应中与不同浓度的壳聚糖进行了比较试验,其结果也证实了上述解释.对这反应的其他一些影响因素也作了试验并给出了结果.
牛血清白蛋白
可见光区分光光度法
反应机理
Zincon
Bovine serum albumin
VIS-spectrophotometry
Reaction mechanism
Abstract
Mechanism of the interaction between the reagent zincon and bovine serum albumin (BSA) was studied by spectrophotometry in visible spectral region with variation of concentration of BSA.Based on the spectral change of the reaction system at pH 4.2,with decrease of absorbance at 465 nm (λmax of zincon) and increase of absorbance at 2 new peak wavelengths of 564 nm and 600 nm with increase of concentration of BSA,it was concluded that a compound complex was formed between the 2 reactants.It was explicated that both the electrostatic effect and hydrophobic effect played important role in the formation of the zincon-BSA compound complex,with the latter as leading role.It was further proved by the reaction between zincon and BSA in comparison with chitosan of different concentrations.Some other influential factors of the reaction were also tested and results given.
中图分类号 O657.32
所属栏目 试验与研究
基金项目 安徽省教育厅自然科学研究重点项目(KJ2007A131ZC);宿州学院教授/博士基金项目(2006jb05)资助
收稿日期 2007/12/28
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备注曹稳根(1964-),男,安徽东至县人,教授,硕士,主要研究方向为蛋白质和多糖生物化学.
引用该论文: CAO Wen-gen,JIAO Qing-cai. On the Mechanism of the Interaction Between Zincon and Bovine Serum Albumin[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(2): 128～130
曹稳根,焦庆才. 锌试剂与牛血清白蛋白相互作用机理[J]. 理化检验－化学分册, 2009, 45(2): 128～130
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参考文献
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【2】Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Anal Biochem,1976,72:248-254．
【3】Wei Yongju,Li Kean,Tong Shengyang.The interaction of bromophenol blue with protein in acidic solution[J].Talanta,1996,43(1):1-10．
【4】曹稳根,焦庆才,刘茜,等.考马斯亮蓝显色剂变色机理的研究[J].化学学报,2002,60(9):1656-1661．
【5】曹稳根,焦庆才,刘茜.蛋白质三元复合物空间定向作用机理的研究[J].光谱学与光谱分析,2005,25(9):1478-1481．
【6】Cao Wengen,Jiao Qingcai,Fu Yu,et al.Mechanism of the interaction between bromophenol blue and bovine serum albumin[J].Spectroscopy letters,2003,36(3):197-203．
【7】迟燕华,庄稼,李娜,等.锌试剂与牛血清白蛋白作用机理的研究[J].高等学校化学学报,1999,20(11):1697-1702．
【8】Jiao Qingcai,Liu Qian.Characterization of the interaction between methylene blue and glycosaminoglycans[J].Spectrochim Acta Part A,1999,55(7/8):1667-1673．
【9】Liu Qian,Jiao Qingcai,Liu Zhili,et al.The interaction of polysaccharides with a spectroscopy probe: The anion effect on the binding site of heparin[J].Spectroscopy letters,2001,34(1):25-34．
【10】曹稳根,焦庆才,陈雷,等.透明质酸与天青A空间定向相互作用机理[J].南京大学学报:自然科学版,2003,39(4):573-579．
【11】曹稳根,陈雷,焦庆才,等.硫酸软骨素与天青A空间定向相互作用机理的研究[J].光谱学与光谱分析,2003,23(3):587-590．
【12】柯惟中,余多慰,冯玉英.牛血清白蛋白水溶液的紫外吸收光谱和激光喇曼光谱分析[J].光谱学与光谱学分析,1993,13(5):55-58．
【13】Klotz L M,Harris J U.Marcomolecule-small molecule interaction.strong binding by intramolecularly cross-linked polylysine[J].Biochem,1997,10:923．
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