毛细管电泳非接触电导分离检测金属氧化物纳米粒子
Separation and Inspection of Nanoparticles of TiO2 and Al2O3 by Capillary Electrophoresis with Non-contact Conductivity Detection
摘 要
采用毛细管电泳非接触电导检测技术,以碳酸钠-碳酸氢钠为缓冲体系,同时分离测定了纳米二氧化钛和三氧化二铝两种金属氧化物的悬浮粒子.考察了缓冲溶液种类、浓度及pH对分离效果的影响,探讨了激发频率、激发电压对检测信号信噪比的影响.在最佳分离检测条件下,不同粒径的二氧化钛和三氧化二铝悬浮粒子在8 min内可实现完全分离.所选择的最佳条件为:① pH 10.5缓冲溶液为5 mmol·L-1碳酸钠和碳酸氢钠混合溶液;② 分离电压20 kV;③ 进样条件20 kV,6 s;④ 激发频率460 kHz;⑤ 交流激发电压4 V(给出最高的S/N比值).
非接触电导检测
纳米粒子
金属氧化物
Capillary electrophoresis
Non-contact conductivity detection
Nanoparticles
Metal oxides (e.g.TiO2 and Al2O3)
Abstract
Separation and inspection of nanoparticles of TiO2 and Al2O3 in a mixed suspension were performed simultaneously by capillary electrophoresis with non-contact conductivity detection.Various factors,including kinds and concentration of buffer solutions and pH of solution influential to the efficiency of separation,were scrutinized and effects of excitation frequency and alternating current excitation voltage on the signal to noise ratio of measuring signal were also studied.Nanoparticles of TiO2 and Al2O3 of different grain size in a suspension were completely separated within 8 min under the following optimized conditions: ① buffer solution: 5 mmol·L-1 Na2CO3 and NaHCO3 mixed solution of pH 10.5;② separation voltage: 20 kV;③ sample introduction at 20 kV for 6 s;④ excitation frequency: 460 kHz;⑤ AC excitation voltage: 4 V,giving highest value of S/N ratio.
中图分类号 O657.8
所属栏目 试验与研究
基金项目 国家自然科学基金(No.20675036),山东省自然科学基金(Y2006B04),山东省教育厅科技计划项目(J05C08)山东省“泰山学者”建设工程专项经费
收稿日期 2008/4/1
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备注管了(1979-),女,山东青岛人,硕士研究生,主要从事毛细管电泳研究.
引用该论文: GUAN Liao,WANG Li-xin,FU Chong-gang. Separation and Inspection of Nanoparticles of TiO2 and Al2O3 by Capillary Electrophoresis with Non-contact Conductivity Detection[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(2): 131～133
管了,王立新,傅崇岗. 毛细管电泳非接触电导分离检测金属氧化物纳米粒子[J]. 理化检验－化学分册, 2009, 45(2): 131～133
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参考文献
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【2】Mc Cormick R M.Characterization of silica sols using capillary zone electrophoresis[J].Liq Chromatogr,1991,14(5):939-952．
【3】Schnabel U,Fischer C H,Kenndler E.Characterization of colloidal gold nanoparticles according to size by capillary zone electrophoresis[J].J Microcolumn,1997,9(7):529-534．
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